肝癌细胞系中 mda-7/IL-24 受体的表达模式。

Expression pattern of mda-7/IL-24 receptors in liver cancer cell lines.

机构信息

Abdominal Cancer Centre, Third Affiliated Hospital, Kunming Medical College, Kunming 650102, China.

出版信息

Hepatobiliary Pancreat Dis Int. 2009 Aug;8(4):402-6.

PMID:19666410

Abstract

BACKGROUND

The mda-7/IL-24 receptor belongs to the type II cytokine receptor family, and its two heterodimeric receptors are IL-22R1/IL-20R2 and IL-20R1/IL-20R2. Mda-7/IL-24 receptor expression in liver cancer cell lines has not yet been described. This information may be helpful for further clinical gene therapy.

METHODS

With normal skin total RNA as template, the cDNA sequences of IL-20R1, IL-20R2 and IL-22R were amplified by RT-PCR. Total RNA was extracted from cultured liver cancer cell lines and a normal liver cell line, then detected by northern blotting, and the expression of mda-7/IL-24 receptors was analyzed.

RESULTS

PLC/PRF/5 and SMMC-7721 expressed IL-20R1; BEL-7402, Hep3B, HepG2, and PLC/PRF/5 expressed IL-20R2; and HepG2 and PLC/PRF/5 expressed IL-22R. Only HepG2 expressed the IL-22R/IL-20R2 receptor complex. PLC/PRF/5 completely expressed both heterodimeric receptors. Huh-7, QGY-7701 and WRL-68 did not express the IL-24 receptor.

CONCLUSION

Complete mda-7/IL-24 receptors are seldom expressed in liver cancer cell lines.

摘要

背景

mda-7/IL-24 受体属于 II 型细胞因子受体家族，其两个异二聚体受体是 IL-22R1/IL-20R2 和 IL-20R1/IL-20R2。肝癌细胞系中 mda-7/IL-24 受体的表达尚未描述。这些信息可能有助于进一步的临床基因治疗。

方法

以正常皮肤总 RNA 为模板，通过 RT-PCR 扩增 IL-20R1、IL-20R2 和 IL-22R 的 cDNA 序列。从培养的肝癌细胞系和正常肝细胞系中提取总 RNA，通过 northern blot 检测，分析 mda-7/IL-24 受体的表达。

结果

PLC/PRF/5 和 SMMC-7721 表达 IL-20R1；BEL-7402、Hep3B、HepG2 和 PLC/PRF/5 表达 IL-20R2；而 HepG2 和 PLC/PRF/5 表达 IL-22R。只有 HepG2 表达 IL-22R/IL-20R2 受体复合物。PLC/PRF/5 完全表达两种异二聚体受体。Huh-7、QGY-7701 和 WRL-68 不表达 IL-24 受体。