Karim Rooshdiya Z, Li Wei, Sanki Amira, Colman Marjorie H, Yang Yee Hwa, Thompson John F, Scolyer Richard A
Discipline of Pathology, The University of Sydney, Sydney Melanoma Unit, Sydney Cancer Centre, Royal Prince Alfred Hospital and Melanoma Institute Australia.
Int J Surg Pathol. 2009 Oct;17(5):361-7. doi: 10.1177/1066896909336177. Epub 2009 Aug 10.
The immunohistochemical expression of cell cycle proteins p16, cyclin D1, and pRb was assessed in 112 benign and malignant melanocytic tumors and correlated with tumor progression, prognosis, and outcome. Comparing benign and malignant tumors, there were significant differences in the median score for all 3 proteins, with decreased p16 (P = .000001), increased cyclin D1 (P = .01), and increased pRb in melanomas (P = .01). There was a progressive loss of expression of p16 with progression from benign naevi to primary melanomas and to metastases. p16 was significantly decreased in primary tumors from melanoma patients who developed recurrent disease (P = .0000013). Cyclin D1 and pRb showed a progressive increase in expression from benign to malignant tumors but with relative decreases in the more advanced tumors (thick primaries and metastatic melanomas). Alterations in cell cycle proteins involved in G1/S transition are implicated in melanocytic tumor progression and have a potential role in diagnosis and prognostication.
在112例良性和恶性黑素细胞肿瘤中评估了细胞周期蛋白p16、细胞周期蛋白D1和磷酸化视网膜母细胞瘤蛋白（pRb）的免疫组化表达，并将其与肿瘤进展、预后和结局相关联。比较良性和恶性肿瘤，所有3种蛋白的中位评分存在显著差异，黑色素瘤中p16降低（P = 0.000001）、细胞周期蛋白D1升高（P = 0.01）、pRb升高（P = 0.01）。随着从良性痣进展为原发性黑色素瘤再到转移灶，p16的表达逐渐丧失。在发生复发性疾病的黑色素瘤患者的原发性肿瘤中，p16显著降低（P = 0.0000013）。细胞周期蛋白D1和pRb从良性肿瘤到恶性肿瘤表达逐渐增加，但在更晚期肿瘤（厚的原发性肿瘤和转移性黑色素瘤）中相对降低。参与G1/S转换的细胞周期蛋白改变与黑素细胞肿瘤进展有关，在诊断和预后方面具有潜在作用。
