Kim Chang-Hee, Kim Hye-Young, O Chang Sun, Oh Seung-Ha, Lee Ji-Eun, Lee Jun Ho
Department of Otorhinolaryngology, Seoul National University College of Medicine, Seoul National University Hospital and Sensory Organ Research Institute, Seoul National University Medical Research Center, Seoul 110-744, Korea.
Neuroreport. 2009 Sep 23;20(14):1275-8. doi: 10.1097/WNR.0b013e32833017eb.
The purpose of this study was to investigate the developmental change of epithelial Na channel function in epithelial cells of rat Reissner's membrane. The Reissner's membrane was isolated from the upper turn of the cochlea at different stages of development. The vibrating probe technique was chosen to measure transepithelial currents under short circuit conditions. Amiloride-sensitive Na current in the Reissner's membrane developed postnatally between days 3 and 5, which was completely blocked by amiloride. This tendency remained from days 5 through 14. Development of Na transport in epithelial cells of the Reissner's membrane may contribute considerably to the accomplishment of low Na concentration in the endolymph during early neonatal period.
本研究的目的是调查大鼠内淋巴囊膜上皮细胞中上皮钠通道功能的发育变化。在内淋巴囊膜发育的不同阶段，从耳蜗上半圈分离出内淋巴囊膜。选择振动探针技术来测量短路条件下的跨上皮电流。内淋巴囊膜中的氨氯地平敏感钠电流在出生后第3至5天出现，完全被氨氯地平阻断。这种趋势从第5天持续到第14天。内淋巴囊膜上皮细胞中钠转运的发育可能在很大程度上有助于新生儿早期内淋巴中低钠浓度的形成。
