Departamento de Química Analítica, Universidad de Córdoba, Córdoba, Spain.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2009 Feb;26(2):265-74. doi: 10.1080/02652030802368740.
Decanoic acid reverse micelle-based coacervates were used to provide simple, rapid and almost solventless extraction of bisphenol A (BPA) from canned fatty foods. The procedure involved the extraction of 200-400 mg of homogenised food sample with an aqueous solution containing 20% THF and 200 mg of decanoic acid, conditions under which the coacervate (around 550 microl) formed in situ and instantaneously. The overall sample treatment took about 30 min and several samples could be simultaneously treated using conventional laboratory equipment. No clean-up or solvent evaporation were required before determination of BPA by liquid chromatography and fluorescence detection. Recoveries in samples were between 90 and 99%, with relative standard deviations in the range 2-7%. The limit of quantification ranged 29-15 ng g(-1) for 200-400 mg of sample, being far below the current specific migration limit (SML) set by the European Commission (600 ng g(-1)). The method was successfully applied to the determination of BPA in the solid content of canned fish (from 20 to 129 ng g(-1)) and meat (from undetected to 37 ng g(-1)).
癸酸反胶束共凝聚物被用于提供简单、快速且几乎无溶剂的方法，从罐装脂肪食品中提取双酚 A（BPA）。该方法涉及用含有 20%THF 和 200mg 癸酸的水溶液提取 200-400mg 匀浆食品样品，在这种条件下，共凝聚物（约 550μl）就地即时形成。整个样品处理过程约需 30min，使用常规实验室设备可以同时处理多个样品。在通过液相色谱和荧光检测测定 BPA 之前，无需进行净化或溶剂蒸发。在 200-400mg 样品中，回收率在 90-99%之间，相对标准偏差在 2-7%范围内。对于 200-400mg 样品，定量限范围为 29-15ng/g，远低于欧盟委员会设定的当前特定迁移限量（SML）（600ng/g）。该方法成功应用于测定罐装鱼（20-129ng/g）和肉（未检出至 37ng/g）的固体含量中的 BPA。
