Linacre A, Kellett E, Saunders S, Bright K, Benjamin P R, Burke J F
Department of Biochemistry, School of Biological Sciences, Brighton, East Sussex, United Kingdom.
J Neurosci. 1990 Feb;10(2):412-9. doi: 10.1523/JNEUROSCI.10-02-00412.1990.
The neuropeptide Phe-Met-Arg-Phe-NH2 (FMRFamide) is a potent cardioactive neuropeptide in Lymnaea stagnalis. Isolation and sequencing of 2 cDNAs and a genomic clone shows that a single gene encodes a precursor protein which contains 9 copies of the FMRFamide peptide, 2 copies of the related peptide Phe-Leu-Arg-Phe-NH2 (FLRFamide), and single copies of the putative pentapeptides Gln-Phe-Tyr-Arg-lle-NH2 (posttranslationally modified to pQFYRlamide) and Glu-Phe-Leu-Arg-lle-NH2 (EFLRlamide). The gene is transcribed in the CNS and gives rise to a single RNA of 1.7 kb in size. The organization of the Lymnaea gene is significant with respect to the evolution of FMRFamide and related peptides in other organisms.
神经肽苯丙氨酸-甲硫氨酸-精氨酸-苯丙氨酸-酰胺（FMRF酰胺）是椎实螺中的一种强效心脏活性神经肽。两个cDNA和一个基因组克隆的分离与测序表明，单个基因编码一种前体蛋白，该前体蛋白包含9个FMRF酰胺肽拷贝、2个相关肽苯丙氨酸-亮氨酸-精氨酸-苯丙氨酸-酰胺（FLRF酰胺）拷贝，以及假定的五肽谷氨酰胺-苯丙氨酸-酪氨酸-精氨酸-异亮氨酸-酰胺（翻译后修饰为pQFYR酰胺）和谷氨酸-苯丙氨酸-亮氨酸-精氨酸-异亮氨酸-酰胺（EFLR酰胺）的单拷贝。该基因在中枢神经系统中转录，产生一个大小为1.7 kb的单一RNA。就其他生物体中FMRF酰胺及相关肽的进化而言，椎实螺基因的组织方式具有重要意义。
