School of Pharmacy, Second Military Medical University, Shanghai, China.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2009 May;26(5):595-603. doi: 10.1080/02652030802641880.
Adulteration of botanical dietary supplements with prohibited synthetic drugs has become a serious problem. In this paper, a method for testing synthetic drugs used to adulterate botanical dietary supplements was developed using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) coupled with a linearity ion-trap system in the multiple reaction monitoring (MRM) plus enhanced product ion (EPI) mode. Twenty-three drugs exhibiting various pharmacological effects, comprising blood pressure and lipid-lowering agents, sedative drugs, anti-diabetic drugs, weight-reducing agents and aphrodisiac compounds, were studied. For all drugs, a single transition was monitored using protonated molecules as precursor ions. EPI spectra were stored in a library and recognized by library searching. Several undeclared drugs were identified in herbal remedies, e.g., glibenclamide, sibutramine hydrochloride and sildenafil. Overall, 35 positive samples were found out of a total of 105 botanical dietary supplements tested. The method was selective, sensitive, rapid, high-throughput and reliable.
植物性膳食补充剂中掺假禁用合成药物已成为一个严重的问题。本文采用液相色谱-电喷雾电离-串联质谱（LC-ESI-MS/MS）技术，结合线性离子阱系统，在多重反应监测（MRM）加增强子离子（EPI）模式下，建立了一种检测用于掺假植物性膳食补充剂的合成药物的方法。研究了 23 种具有不同药理作用的药物，包括降压降脂药、镇静药、抗糖尿病药、减肥药和壮阳药。对于所有药物，均采用质子化分子作为前体离子进行单重反应监测。EPI 图谱被储存在一个文库中，并通过库检索进行识别。在草药制剂中发现了几种未申报的药物，如格列本脲、盐酸西布曲明和西地那非。在总共检测的 105 种植物性膳食补充剂中，发现了 35 种阳性样品。该方法具有选择性、灵敏度高、快速、高通量和可靠的特点。
