[组织转谷氨酰胺酶在肾间质纤维化大鼠中的表达及银杏叶提取物的干预作用]
[Expression of tissue transglutaminase on renal interstitial fibrosis rats and intervention of GBE].
作者信息
Wang Dan, Li Xiangjun, Yu Xiaoyan, Shi Yan, Yin Li
机构信息
Department of Experimental Pharmacology and Toxicology, School of Pharmacy, Jilin University, Changchun 130021, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2009 May;34(9):1133-6.
OBJECTIVE
To investigate the expression of tissue transglutaminase( tTG) in unilateral ureteral obstruction (UUO) rats and the intervention of Folium Ginkgo (GBE).
METHOD
The animal models of unilateral ureteral obstruction (UUO) were used. Wistar rats were randomly divided into four groups, the Sham-operated group, the UUO group, the benazepril (Bena)-treated UUO group and the GBE-treated group. The rats were sacrificed at day 14. Histological changes in renal tubular interstitium were observed with HE and Masson staining, and the mRNA and protein levels of tTG and FN were detected by RT-PCR and immunohistochemistry.
RESULT
Compared with the sham group, the expression of tTG and FN were significantly increased (P < 0.01), and decreased after been treated by Bena and GBE (P < 0.05).
CONCLUSION
Bena and GBE may suppress the development of fibrosis partially via down-regulation of tTG expression.
目的
探讨组织转谷氨酰胺酶（tTG）在单侧输尿管梗阻（UUO）大鼠中的表达及银杏叶提取物（GBE）的干预作用。
方法
采用单侧输尿管梗阻动物模型。将Wistar大鼠随机分为四组，即假手术组、UUO组、苯那普利（Bena）治疗的UUO组和GBE治疗组。于第14天处死大鼠。用HE和Masson染色观察肾小管间质的组织学变化，采用RT-PCR和免疫组化检测tTG和FN的mRNA及蛋白水平。
结果
与假手术组相比，tTG和FN的表达显著增加（P < 0.01），经Bena和GBE治疗后表达降低（P < 0.05）。
结论
Bena和GBE可能通过下调tTG表达部分抑制纤维化的发展。
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