Theodore Harcke H, Monaghan Timothy, Yee Nicole, Finelli Louis
Armed Forces Institute of Pathology and Uniformed Services University of the Health Sciences, Wilmington, DE 19899, USA.
J Forensic Sci. 2009 Sep;54(5):1123-6. doi: 10.1111/j.1556-4029.2009.01114.x. Epub 2009 Aug 3.
Our objective is to document the recovery of DNA from the spinal cord or surrounding dura mater in 11 cases of severely burned human remains. Radiographs established that portions of charred tissue contained spine segments. Multidetector computed tomography (MDCT) revealed that each spine specimen contained an intact spinal cord remnant. A full DNA profile was obtained from seven specimens using spinal cord dura mater in six specimens and spinal cord medulla in one specimen. A partial profile was obtained from four specimens (spinal cord dura mater, 2; spinal cord medulla, 2). Bone and muscle surrounding the spinal cord appear to insulate nucleic acid containing tissue from critical thermal degradation. The spinal cord, which is easily identified by MDCT examination of remains and easily recovered at the postmortem examination, can be a source of DNA with extraction yields comparable with other tissue sources. Specimens of dura mater are preferable as processing time is faster than bone.
我们的目标是记录在11例严重烧伤遗体中从脊髓或周围硬脑膜中提取DNA的情况。X光片显示烧焦组织的部分包含脊柱节段。多排螺旋计算机断层扫描（MDCT）显示每个脊柱标本都含有完整的脊髓残余物。使用6个标本中的脊髓硬脑膜和1个标本中的脊髓髓质，从7个标本中获得了完整的DNA图谱。从4个标本（脊髓硬脑膜，2个；脊髓髓质，2个）中获得了部分图谱。脊髓周围的骨骼和肌肉似乎能使含核酸的组织免受严重热降解。通过对遗体进行MDCT检查很容易识别脊髓，并且在尸检时很容易获取，脊髓可以作为DNA的来源，提取产量与其他组织来源相当。硬脑膜标本更可取，因为处理时间比骨骼更快。
