Université de Technologie de Compiègne - CNRS UMR 6600 Laboratoire de Biomécanique et Bioingénierie, BP 20529, 60205 Compiègne Cedex, France.
Chem Phys Lipids. 2009 Nov;162(1-2):17-24. doi: 10.1016/j.chemphyslip.2009.08.002. Epub 2009 Aug 21.
The interaction of cytochrome c (cyt c) with fluid/gel neutral supported lipid membranes was investigated by time-lapse atomic force microscopy (AFM). AFM revealed the random formation of depressed areas in fluid membranes promoted by cyt c. These depressions corresponded to the desorption of fluid bilayer patches induced by cyt c. By contrast, the gel domains were never desorbed but they were progressively thickened in the presence of the protein. These results suggest that cyt c molecules might intercalate between the mica and the lipid bilayer. Although the interaction of cyt c with the mica surface is likely to be an artifact, this work is the first direct observation of cyt c ability to cross membranes. Furthermore, our data show that the net positive charge of cyt c molecules plays a pivotal role but it is not the sole factor responsible for cyt c insertion in the membrane.
通过实时原子力显微镜（AFM）研究细胞色素 c（cyt c）与流体/凝胶中性支撑脂质膜的相互作用。AFM 揭示了 cyt c 促进流体膜中凹陷区域的随机形成。这些凹陷对应于 cyt c 诱导的流体双层片的解吸。相比之下，凝胶区域从未被解吸，但在蛋白质存在下逐渐增厚。这些结果表明 cyt c 分子可能插入云母和脂质双层之间。尽管 cyt c 与云母表面的相互作用可能是一种假象，但这项工作首次直接观察到 cyt c 穿过膜的能力。此外，我们的数据表明 cyt c 分子的净正电荷起着关键作用，但不是 cyt c 插入膜的唯一因素。
