[一种改良的液氮冷冻法建立犬股骨头坏死模型]
[An improved liquid nitrogen freezing method for establishing canine models of femoral head necrosis].
作者信息
Liu Guo-zhu, Yang Tong-tao, Han Jian-wei, Wu Jin, Wen Yan-hua, Gao Jie, Ma Bao-an
机构信息
Department of Orthopedics, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2009 Aug;29(8):1602-4.
OBJECTIVE
To establish an canine model of femoral head osteonecrosis using an improved liquid nitrogen freezing method.
METHODS
Sixteen adult canines were divided into 4 groups at random and subjected to instant freezing of the unilateral femoral head with liquid nitrogen. The dogs were observed at 2, 4, 8, and 16 weeks after the operation for radiographic, gross and pathological changes of the femoral head.
RESULTS
Significant radiographic, gross and pathological changes occurred in the femoral head after the freezing.
CONCLUSION
Improved liquid nitrogen freezing of the femoral head provides a simple and convenient method for establishing animal models of femoral head osteonecrosis.
目的
采用改良液氮冷冻法建立犬股骨头坏死模型。
方法
将16只成年犬随机分为4组，对单侧股骨头进行液氮即刻冷冻。术后2、4、8和16周观察犬股骨头的影像学、大体及病理变化。
结果
冷冻后股骨头出现明显的影像学、大体及病理变化。
结论
改良的股骨头液氮冷冻法为建立股骨头坏死动物模型提供了一种简单便捷的方法。
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