Liu Yun, Zhang Li, Desiderio Stephen
Department of Molecular Biology and Genetics, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Adv Exp Med Biol. 2009;650:157-65. doi: 10.1007/978-1-4419-0296-2_13.
In the course of lymphoid development, V(D)J recombination is subject to stringent locus-specific and temporal regulation. These constraints are ultimately responsible for several features peculiar to lymphoid development, including the lineage specificity of antigen receptor assembly, allelic exclusion and receptor editing. In addition, cell cycle phase-dependent regulation of V(D)J recombinase activity ensures that DNA rearrangement is completed by the appropriate mechanism of DNA repair. Regulation of V(D)J recombination involves interactions between the V(D)J recombinase--a heteromeric complex consisting of RAG-1 and RAG-2 subunits--and macromolecular assemblies extrinsic to the recombinase. This chapter will focus on those features of the recombinase itself--and in particular the RAG-2 subunit--that interact with extrinsic factors to establish patterns of temporal control and locus specificity in developing lymphocytes.
在淋巴细胞发育过程中，V(D)J重组受到严格的基因座特异性和时间调控。这些限制最终导致了淋巴细胞发育特有的几个特征，包括抗原受体组装的谱系特异性、等位基因排斥和受体编辑。此外，V(D)J重组酶活性的细胞周期阶段依赖性调控确保了DNA重排通过适当的DNA修复机制完成。V(D)J重组的调控涉及V(D)J重组酶（一种由RAG-1和RAG-2亚基组成的异源复合物）与重组酶外部的大分子组装体之间的相互作用。本章将重点关注重组酶本身的那些特征，特别是RAG-2亚基，它与外部因子相互作用，在发育中的淋巴细胞中建立时间控制和基因座特异性模式。
