评估用于 PC-IDMS 的 ALiPHAT 方法以及检测限与非极性表面积的相关性。
Evaluation of the ALiPHAT method for PC-IDMS and correlation of limits-of-detection with nonpolar surface area.
机构信息
W. M. Keck FT-ICR Mass Spectrometry Laboratory, Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA.
出版信息
J Am Soc Mass Spectrom. 2009 Nov;20(11):2006-12. doi: 10.1016/j.jasms.2009.07.019. Epub 2009 Aug 7.
Abstract
PC-IDMS experiments for two peptides, laminin nonapeptide and the N-terminal tryptic peptide of prostate specific antigen, were performed utilizing a variety of alkylating reagents. These experiments were conducted to investigate how hydrophobicity influences the limits-of-detection (LOD) by altering their electrospray ionization response. Nonpolar surface areas were calculated for both peptides and all alkylating reagents to provide an estimate of the hydrophobicity of the differently alkylated peptides. Decreases in LOD by 2-fold were observed for both peptides between the best and worst performing combination of alkylating reagent. However, while an increase in hydrophobicity was found to aid in decreasing LOD to an extent, beyond a certain hydrophobicity, we observed a decrease.
摘要
针对两种肽（层粘连蛋白九肽和前列腺特异性抗原的 N 端胰蛋白酶肽）进行了 PC-IDMS 实验，使用了多种烷化试剂。这些实验旨在通过改变它们的电喷雾电离响应来研究疏水性如何影响检测限（LOD）。为两种肽和所有烷化试剂计算了非极性表面积，以提供对不同烷化肽疏水性的估计。在最佳和最差烷化试剂组合之间，两种肽的 LOD 均降低了 2 倍。然而，虽然发现疏水性的增加有助于在一定程度上降低 LOD，但超过一定的疏水性后，我们观察到 LOD 降低。
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本文引用的文献
1
Ion formation from charged droplets: Roles of geometry, energy, and time.带电液滴的离子形成：几何形状、能量和时间的作用。
J Am Soc Mass Spectrom. 1993 Jul;4(7):524-35. doi: 10.1016/1044-0305(93)85014-O.
2
Absolute quantification of C-reactive protein in human plasma derived from patients with epithelial ovarian cancer utilizing protein cleavage isotope dilution mass spectrometry.利用蛋白质裂解同位素稀释质谱法对上皮性卵巢癌患者血浆中C反应蛋白进行绝对定量分析。
J Proteome Res. 2009 Feb;8(2):1085-90. doi: 10.1021/pr800922p.
3
Synthesis, characterization, and application of iodoacetamide derivatives utilized for the ALiPHAT strategy.用于ALiPHAT策略的碘乙酰胺衍生物的合成、表征及应用
J Am Chem Soc. 2008 Feb 20;130(7):2122-3. doi: 10.1021/ja076849y. Epub 2008 Jan 26.
4
Achieving augmented limits of detection for peptides with hydrophobic alkyl tags.实现对带有疏水烷基标签的肽段检测限的提高。
Anal Chem. 2007 Jun 1;79(11):3989-95. doi: 10.1021/ac070558q. Epub 2007 May 4.
5
Enhancing electrospray ionization efficiency of peptides by derivatization.通过衍生化提高肽的电喷雾电离效率。
Anal Chem. 2006 Jun 15;78(12):4175-83. doi: 10.1021/ac0602266.
6
Derivatization by 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate for enhancing the ionization yield of small peptides and glycopeptides in matrix-assisted laser desorption/ionization and electrospray ionization mass spectrometry.通过6-氨基喹啉-N-羟基琥珀酰亚胺基氨基甲酸酯衍生化以提高基质辅助激光解吸/电离和电喷雾电离质谱中小肽和糖肽的电离产率。
Rapid Commun Mass Spectrom. 2006;20(9):1469-79. doi: 10.1002/rcm.2464.
7
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J Mass Spectrom. 2006 May;41(5):623-32. doi: 10.1002/jms.1020.
8
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Nat Chem Biol. 2005 Oct;1(5):252-62. doi: 10.1038/nchembio736.
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Expert Rev Proteomics. 2004 Dec;1(4):421-31. doi: 10.1586/14789450.1.4.421.
10
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J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Dec 25;813(1-2):1-26. doi: 10.1016/j.jchromb.2004.09.057.
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