评估用于 PC-IDMS 的 ALiPHAT 方法以及检测限与非极性表面积的相关性。
Evaluation of the ALiPHAT method for PC-IDMS and correlation of limits-of-detection with nonpolar surface area.
机构信息
W. M. Keck FT-ICR Mass Spectrometry Laboratory, Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA.
出版信息
J Am Soc Mass Spectrom. 2009 Nov;20(11):2006-12. doi: 10.1016/j.jasms.2009.07.019. Epub 2009 Aug 7.
Abstract
PC-IDMS experiments for two peptides, laminin nonapeptide and the N-terminal tryptic peptide of prostate specific antigen, were performed utilizing a variety of alkylating reagents. These experiments were conducted to investigate how hydrophobicity influences the limits-of-detection (LOD) by altering their electrospray ionization response. Nonpolar surface areas were calculated for both peptides and all alkylating reagents to provide an estimate of the hydrophobicity of the differently alkylated peptides. Decreases in LOD by 2-fold were observed for both peptides between the best and worst performing combination of alkylating reagent. However, while an increase in hydrophobicity was found to aid in decreasing LOD to an extent, beyond a certain hydrophobicity, we observed a decrease.
摘要
针对两种肽（层粘连蛋白九肽和前列腺特异性抗原的 N 端胰蛋白酶肽）进行了 PC-IDMS 实验，使用了多种烷化试剂。这些实验旨在通过改变它们的电喷雾电离响应来研究疏水性如何影响检测限（LOD）。为两种肽和所有烷化试剂计算了非极性表面积，以提供对不同烷化肽疏水性的估计。在最佳和最差烷化试剂组合之间，两种肽的 LOD 均降低了 2 倍。然而，虽然发现疏水性的增加有助于在一定程度上降低 LOD，但超过一定的疏水性后，我们观察到 LOD 降低。
相似文献
1
Evaluation of the ALiPHAT method for PC-IDMS and correlation of limits-of-detection with nonpolar surface area.
J Am Soc Mass Spectrom. 2009 Nov;20(11):2006-12. doi: 10.1016/j.jasms.2009.07.019. Epub 2009 Aug 7.
2
Improving limits of detection for B-type natriuretic peptide using PC-IDMS: an application of the ALiPHAT strategy.
Analyst. 2010 Jan;135(1):36-41. doi: 10.1039/b919484c. Epub 2009 Nov 19.
3
Absolute quantification of the model biomarker prostate-specific antigen in serum by LC-Ms/MS using protein cleavage and isotope dilution mass spectrometry.
J Proteome Res. 2004 May-Jun;3(3):644-52. doi: 10.1021/pr049963d.
4
Evaluating nonpolar surface area and liquid chromatography/mass spectrometry response: an application for site occupancy measurements for enzyme intermediates in polyketide biosynthesis.
Rapid Commun Mass Spectrom. 2014 Dec 15;28(23):2511-22. doi: 10.1002/rcm.7051.
5
Amino Acid Analysis by Hydrophilic Interaction Chromatography Coupled with Isotope Dilution Mass Spectrometry.
Methods Mol Biol. 2019;2030:111-118. doi: 10.1007/978-1-4939-9639-1_9.
6
Alkylating tryptic peptides to enhance electrospray ionization mass spectrometry analysis.
Anal Chem. 2010 Dec 15;82(24):10135-42. doi: 10.1021/ac1019792. Epub 2010 Nov 29.
7
Liquid chromatography-tandem mass spectrometry approach for quantification of mucins from sputum using 13C,15N-labeled peptides as internal standards.
Anal Biochem. 2013 Mar 1;434(1):84-92. doi: 10.1016/j.ab.2012.10.033. Epub 2012 Nov 5.
8
Selection of possible signature peptides for the detection of bovine lactoferrin in infant formulas by LC-MS/MS.
PLoS One. 2017 Sep 19;12(9):e0184152. doi: 10.1371/journal.pone.0184152. eCollection 2017.
9
The effects of isotope-labeled analogs on the LC-IDMS measurement by comparison of ESI responses and matrix effect of melamine, C-melamine, C+N-melamine, and N-melamine.
Anal Bioanal Chem. 2017 May;409(12):3233-3243. doi: 10.1007/s00216-017-0263-4. Epub 2017 Feb 27.
10
Application of amino acid analysis using hydrophilic interaction liquid chromatography coupled with isotope dilution mass spectrometry for peptide and protein quantification.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Oct 1;877(27):3059-64. doi: 10.1016/j.jchromb.2009.07.027. Epub 2009 Jul 24.
引用本文的文献
1
New ionization tags based on the structure of the 5-azoniaspiro[4.4]nonyl tag for a sensitive peptide sequencing by mass spectrometry.
Anal Bioanal Chem. 2018 Feb;410(4):1311-1321. doi: 10.1007/s00216-017-0771-2. Epub 2017 Dec 6.
2
Evaluating nonpolar surface area and liquid chromatography/mass spectrometry response: an application for site occupancy measurements for enzyme intermediates in polyketide biosynthesis.
Rapid Commun Mass Spectrom. 2014 Dec 15;28(23):2511-22. doi: 10.1002/rcm.7051.
3
Hydrophobic derivatization of N-linked glycans for increased ion abundance in electrospray ionization mass spectrometry.
J Am Soc Mass Spectrom. 2011 Aug;22(8):1309-17. doi: 10.1007/s13361-011-0140-x. Epub 2011 May 3.
4
Alkylating tryptic peptides to enhance electrospray ionization mass spectrometry analysis.
Anal Chem. 2010 Dec 15;82(24):10135-42. doi: 10.1021/ac1019792. Epub 2010 Nov 29.
5
Interplay of permanent charge and hydrophobicity in the electrospray ionization of glycans.
Anal Chem. 2010 Aug 1;82(15):6636-42. doi: 10.1021/ac101227a.
6
Improving limits of detection for B-type natriuretic peptide using PC-IDMS: an application of the ALiPHAT strategy.
Analyst. 2010 Jan;135(1):36-41. doi: 10.1039/b919484c. Epub 2009 Nov 19.
本文引用的文献
1
Ion formation from charged droplets: Roles of geometry, energy, and time.
J Am Soc Mass Spectrom. 1993 Jul;4(7):524-35. doi: 10.1016/1044-0305(93)85014-O.
2
Absolute quantification of C-reactive protein in human plasma derived from patients with epithelial ovarian cancer utilizing protein cleavage isotope dilution mass spectrometry.
J Proteome Res. 2009 Feb;8(2):1085-90. doi: 10.1021/pr800922p.
3
Synthesis, characterization, and application of iodoacetamide derivatives utilized for the ALiPHAT strategy.
J Am Chem Soc. 2008 Feb 20;130(7):2122-3. doi: 10.1021/ja076849y. Epub 2008 Jan 26.
4
Achieving augmented limits of detection for peptides with hydrophobic alkyl tags.
Anal Chem. 2007 Jun 1;79(11):3989-95. doi: 10.1021/ac070558q. Epub 2007 May 4.
5
Enhancing electrospray ionization efficiency of peptides by derivatization.
Anal Chem. 2006 Jun 15;78(12):4175-83. doi: 10.1021/ac0602266.
6
Derivatization by 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate for enhancing the ionization yield of small peptides and glycopeptides in matrix-assisted laser desorption/ionization and electrospray ionization mass spectrometry.
Rapid Commun Mass Spectrom. 2006;20(9):1469-79. doi: 10.1002/rcm.2464.
7
Derivatisation of arginine residues with malondialdehyde for the analysis of peptides and protein digests by LC-ESI-MS/MS.
J Mass Spectrom. 2006 May;41(5):623-32. doi: 10.1002/jms.1020.
8
Mass spectrometry-based proteomics turns quantitative.
Nat Chem Biol. 2005 Oct;1(5):252-62. doi: 10.1038/nchembio736.
9
Isotope-differentiated binding energy shift tags (IDBEST) for improved targeted biomarker discovery and validation.
Expert Rev Proteomics. 2004 Dec;1(4):421-31. doi: 10.1586/14789450.1.4.421.
10
Current chemical tagging strategies for proteome analysis by mass spectrometry.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Dec 25;813(1-2):1-26. doi: 10.1016/j.jchromb.2004.09.057.
Zotero 插件