Szyf M, Tanigawa G, McCarthy P L
Department of Genetics, Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts.
Mol Cell Biol. 1990 Aug;10(8):4396-400. doi: 10.1128/mcb.10.8.4396-4400.1990.
Although DNA can be extensively methylated de novo when introduced into pluripotent cells, the CpG island in the Thy-1 gene does not become methylated either in the mouse embryo or in embryonic stem cells. A 214-base-pair region near the promoter of the Thy-1 gene protects itself as well as heterologous DNA sequences from de novo methylation. We propose that this nucleotide sequence is representative of a class of important signals that limits de novo methylation in the embryo and establishes the pattern of hypomethylated CpG dinucleotides found in somatic tissues.
尽管DNA导入多能细胞时可大量从头甲基化，但Thy-1基因中的CpG岛在小鼠胚胎或胚胎干细胞中均未发生甲基化。Thy-1基因启动子附近一个214碱基对的区域可保护自身及异源DNA序列不发生从头甲基化。我们认为，该核苷酸序列代表了一类重要信号，这类信号限制胚胎中的从头甲基化，并建立了体细胞组织中低甲基化CpG二核苷酸的模式。
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