硫酸化多糖可预防仓鼠因人类白细胞弹性蛋白酶诱导的急性肺损伤和肺气肿。

Sulfated polysaccharides prevent human leukocyte elastase-induced acute lung injury and emphysema in hamsters.

作者信息

Rao N V, Kennedy T P, Rao G, Ky N, Hoidal J R

机构信息

Division of Pulmonary Medicine, University of Tennessee, Memphis.

出版信息

Am Rev Respir Dis. 1990 Aug;142(2):407-12. doi: 10.1164/ajrccm/142.2.407.

DOI:10.1164/ajrccm/142.2.407

PMID:1974403

Abstract

Studies were designed to explore the possibility that sulfated polysaccharides had the potential to prevent human leukocyte elastase (HLE)-induced lung injury. Arteparon (GAGPS), heparin, heparan sulfate, chondroitin sulfate, and dextran sulfate, but not dextran, inhibited HLE-mediated hydrolysis of succinyl-ala2-val-pNA. GAGPS, used as a paradigmatic sulfated polysaccharide, was a potent inhibitor of elastolysis in vitro. GAGPS given intratracheally prevented acute injury and emphysema in hamsters when administered up to 8 h before HLE insufflation. The results suggest that sulfated polysaccharides may be potent inhibitors of HLE-mediated lung injury.

摘要

开展了多项研究以探索硫酸化多糖是否有可能预防人白细胞弹性蛋白酶（HLE）诱导的肺损伤。阿替派隆（GAGPS）、肝素、硫酸乙酰肝素、硫酸软骨素和硫酸葡聚糖（而非葡聚糖）可抑制HLE介导的琥珀酰 - 丙氨酸2 - 缬氨酸 - 对硝基苯胺水解。作为典型硫酸化多糖的GAGPS在体外是弹性蛋白水解的有效抑制剂。在向仓鼠气管内注入HLE前8小时内给予GAGPS，可预防急性损伤和肺气肿。结果表明，硫酸化多糖可能是HLE介导的肺损伤的有效抑制剂。