Hu Sen, Zhang Li-jian, Bai Hui-ying, Bao Cheng-mei
Burns Institute, First Affiliated Hospital of PLA General Hospital, Beijing, China.
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2009 Sep;21(9):552-4.
To investigate the effect of carbachol (CAR) on visceral perfusion and lipid oxidation injury in rats with sepsis.
Sixty-four Sprague-Dawley (SD) rats received cecal ligation and puncture (CLP) surgery, and they were divided randomly into two groups: septic model group (CLP group, n=32) and septic model with CAR-treatment group (CAR group, n=32). CAR (10 microg/kg, CAR group) or normal saline (CLP group) was immediately injected into penial vein. Sixteen animals in each group were used to observe the mortality rates 12 hours and 24 hours after CLP, and the remaining rats for measurement of variables of blood and tissue. At the 18 hours after CLP, the mean arterial pressure (MAP), the blood flow (BF) of liver, kidney and jejunum, the plasma levels of alanine aminotransferase (ALT) and creatinine (Cr) were measured. Animals were sacrificed after the aforementioned determinations, and specimens of liver, kidney and jejunum were harvested for evaluation of malondialdehyde (MDA), xanthine oxidase (XOD), and assessment of tissue water content (ratio of dry to wet weight) of those organs . The activity of diamine oxidase (DAO) in jejunal tissue was detected.
The mortality rates of 12 hours and 24 hours of CAR group were 25.0% (4/16)and 50.0% (8/16) respectively, all significantly lower than those of CLP group [37.5% (6/16) and 75%(12/16), both P<0.05]. CAR treatment did not result in significant statistical difference in the levels of MAP compared with CLP group at 18 hours after CLP (P>0.05), but led to significant increases in BF of CAR group in liver, kidney and jejunum compared with those of CLP group (all P<0.05). The levels of XOD and MDA, as well as the tissue water content were significantly lower in CAR group than CLP group in kidney and jejunum (all P<0.05). The parameters of organ function were significantly different in CAR group compared with CLP group [ALT: (64.3+/- 8.3) U/L vs. (81.5+/-7.9) U/L, Cr: (96.4+/-7.0) micromol/L vs. (117.1+/-6.7) micromol/L, DAO: (0.20+/- 0.04) U/L vs. (0.12+/-0.03) U/L, all P<0.05].
The results indicate that CAR promotes visceral perfusion, inhibits lipid peroxidation production and alleviates visceral edema and dysfunction in rats with sepsis.
探讨卡巴胆碱（CAR）对脓毒症大鼠内脏灌注及脂质氧化损伤的影响。
64只Sprague-Dawley（SD）大鼠行盲肠结扎穿孔（CLP）手术，随机分为两组：脓毒症模型组（CLP组，n = 32）和CAR治疗脓毒症模型组（CAR组，n = 32）。将CAR（10μg/kg，CAR组）或生理盐水（CLP组）立即注入阴茎静脉。每组16只动物用于观察CLP术后12小时和24小时的死亡率，其余大鼠用于测定血液和组织变量。CLP术后18小时，测量平均动脉压（MAP）、肝、肾和空肠的血流量（BF）、血浆丙氨酸转氨酶（ALT）和肌酐（Cr）水平。在上述测定后处死动物，采集肝、肾和空肠标本，评估丙二醛（MDA）、黄嘌呤氧化酶（XOD），并评估这些器官的组织含水量（干重与湿重之比）。检测空肠组织中二胺氧化酶（DAO）的活性。
CAR组12小时和24小时的死亡率分别为25.0%（4/16）和50.0%（8/16），均显著低于CLP组[37.5%（6/16）和75%（12/16），均P<0.05]。与CLP组相比，CLP术后18小时CAR治疗组的MAP水平无显著统计学差异（P>0.05），但CAR组肝、肾和空肠的BF较CLP组显著增加（均P<0.05）。CAR组肾和空肠的XOD、MDA水平以及组织含水量均显著低于CLP组（均P<0.05）。与CLP组相比，CAR组的器官功能参数有显著差异[ALT：（64.3±8.3）U/L对（81.5±7.9）U/L，Cr：（96.4±7.0）μmol/L对（117.1±6.7）μmol/L，DAO：（0.20±0.04）U/L对（0.12±0.03）U/L，均P<0.05]。
结果表明，CAR可促进脓毒症大鼠的内脏灌注，抑制脂质过氧化产物生成，减轻内脏水肿和功能障碍。
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