Suppr超能文献

水飞蓟宾通过抑制前列腺癌细胞中的蛋白质翻译来抑制缺氧诱导因子-1α(HIF-1α)的表达。

Silibinin inhibits expression of HIF-1alpha through suppression of protein translation in prostate cancer cells.

作者信息

Jung Hui-Jung, Park Jong-Wook, Lee Jason S, Lee Seong-Ryong, Jang Byeong-Churl, Suh Seong-Il, Suh Min-Ho, Baek Won-Ki

机构信息

Chronic Disease Research Center, School of Medicine, Keimyung University, Daegu 700-712, Republic of Korea.

出版信息

Biochem Biophys Res Commun. 2009 Dec 4;390(1):71-6. doi: 10.1016/j.bbrc.2009.09.068. Epub 2009 Sep 22.

Abstract

Silibinin is a polyphenolic flavonoid isolated from the milk thistle (Silybum marianum) and is reported to exhibit anticancer properties. Recently, it has been reported that silibinin inhibits hypoxia-inducible factor-1alpha (HIF-1alpha) expression in cancer cells. However, the precise mechanism by which silibinin decreases HIF-1 expression is not fully understood. In this study, silibinin inhibited basal and hypoxia induced expression levels of HIF-1alpha protein in LNCaP and PC-3 prostate cancer cells, while the rate of HIF-1alpha protein degradation and mRNA levels were not affected. We found that the decrease in HIF-1 protein by silibinin correlated with suppression of de novo synthesis of HIF-1alpha protein. Silibinin inhibited global protein synthesis coincided with reduction of eIF4F complex formation and induction of phosphorylation of the translation initiation factor 2alpha (eIF-2alpha) which can cause inhibition of general protein synthesis. These results suggest that silibinin's activity to inhibit HIF-1alpha protein expression is associated with the suppression of global protein translation.

摘要

水飞蓟宾是一种从水飞蓟(奶蓟)中分离出来的多酚类黄酮,据报道具有抗癌特性。最近,有报道称水飞蓟宾可抑制癌细胞中缺氧诱导因子-1α(HIF-1α)的表达。然而,水飞蓟宾降低HIF-1表达的确切机制尚未完全阐明。在本研究中,水飞蓟宾抑制了LNCaP和PC-3前列腺癌细胞中HIF-1α蛋白的基础表达水平以及缺氧诱导的表达水平,而HIF-1α蛋白的降解速率和mRNA水平未受影响。我们发现,水飞蓟宾导致的HIF-1蛋白减少与HIF-1α蛋白从头合成的抑制相关。水飞蓟宾抑制整体蛋白质合成,这与eIF4F复合物形成的减少以及翻译起始因子2α(eIF-2α)磷酸化的诱导同时发生,而eIF-2α磷酸化可导致整体蛋白质合成的抑制。这些结果表明,水飞蓟宾抑制HIF-1α蛋白表达的活性与整体蛋白质翻译的抑制有关。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验