Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.
Chin Med J (Engl). 2009 Sep 20;122(18):2165-8.
Interleukin (IL)-1beta may effectively decrease introcular pressure (IOP) when administered by subconjunctival injection in normal rabbit. However, IL-1beta is a large molecular agent and an inflammation factor. The aim of this study was to evaluate the penetrability of IL-1beta, and the concentrations of both tumor necrosis factor (TNF)-alpha and IL-6 in the aqueous humor of normal rabbits treated with IL-1beta.
A total of 170 rabbits were used in the study and were assigned to several different treatment groups as follows: 125 of the rabbits were assigned to two groups. In one group, 33 rabbits were injected subconjunctivally with IL-1beta and 39 were injected with saline alone. In the other group, 27 rabbits were given eye drops containing IL-1beta (400 ng/ml) and 26 were given saline alone. Aqueous humor (AH) was drawn and the concentration of IL-1beta within the fluid measured. The IOP was measured in another six rabbits after administration of eye drops containing IL-1beta (400 ng/ml). A further 20 rabbits were assigned to 3 groups as follows: eight untreated normal controls; six injected subconjunctivally with IL-1beta; and six injected subconjunctivally with saline alone. AH was drawn and the concentration of TNF-alpha in the fluid was measured. Another 19 rabbits were assigned to 3 groups as follows: seven untreated normal controls; and six injected subconjunctivally with IL-1beta; and six injected subconjunctivally with saline alone. AH was drawn and the concentration of IL-6 in the fluid measured. Measurement of cytokine concentration was by radio-immunoassay in all cases.
The IL-1beta concentration in the AH was higher in those animals in which it had been administered subconjunctivally (P < 0.01). The IL-1beta concentration in the AH of the animals given eye drops was almost the same as that in the controls (P > 0.05). The administration of IL-1beta in the form of eye drops had little effect upon IOP reduction. Lower TNF-alpha concentrations were seen in the AH after the subconjunctival administration of IL-1beta, but the concentration of IL-6 was the same as in the normal controls.
IL-1beta shows good corneal penetrability after subconjunctival injection into normal rabbit eyes. The IOP reduction induced by IL-1beta is unlikely be associated with an inflammatory response.
白细胞介素（IL）-1β经结膜下注射可有效降低正常兔眼内压（IOP）。然而，IL-1β是一种大分子量的炎症因子。本研究旨在评估 IL-1β在正常兔眼内的渗透性，以及经 IL-1β治疗后房水中肿瘤坏死因子（TNF）-α和 IL-6 的浓度。
本研究共使用 170 只兔子，并将其分为以下几组：125 只兔子分为两组。一组 33 只兔子结膜下注射 IL-1β，39 只兔子注射生理盐水。另一组 27 只兔子滴注含 IL-1β（400ng/ml）的眼药水，26 只兔子滴注生理盐水。抽取房水，测量房水中 IL-1β的浓度。6 只兔子滴注含 IL-1β（400ng/ml）的眼药水后测量眼内压。另外 20 只兔子分为三组：8 只未处理的正常对照；6 只结膜下注射 IL-1β；6 只结膜下注射生理盐水。抽取房水，测量房水中 TNF-α的浓度。另外 19 只兔子分为三组：7 只未处理的正常对照；6 只结膜下注射 IL-1β；6 只结膜下注射生理盐水。抽取房水，测量房水中 IL-6 的浓度。所有情况下，细胞因子浓度均采用放射免疫法测量。
结膜下给药的兔子房水中 IL-1β浓度较高（P<0.01）。滴眼后兔子房水中的 IL-1β浓度与对照组几乎相同（P>0.05）。滴眼给予 IL-1β对降低眼压几乎没有影响。结膜下给予 IL-1β后房水中 TNF-α浓度降低，但 IL-6 浓度与正常对照组相同。
IL-1β经结膜下注射进入正常兔眼后具有良好的角膜穿透性。IL-1β 诱导的眼压降低与炎症反应无关。
