Anhui Key Laboratory of Chemo/Biosensing, Anhui Key Laboratory of Functional Molecular Solids, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, PR China.
Talanta. 2009 Nov 15;80(1):202-6. doi: 10.1016/j.talanta.2009.06.050. Epub 2009 Jul 1.
Amino-functionalized luminescent silica nanoparticles (LSNPs) doped with the europium(III) mixed complex, Eu(TTA)(3)phen with 2-thenoyltrifluoroacetone (TTA) and 1,10-phenanthroline(phen) were synthesized successfully using an revised Stöber method. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier-transform infrared (FTIR), and fluorescence spectroscopy were performed for characterizing the synthesized nanoparticles. In the presence of glucose, the fluorescence intensity of the amino-functionalized LSNPs was enhanced due to the enhanced fluorescence resonance energy transfer. Based on fluorescence-enhancing effect, a simple and sensitive method for the determination of glucose was proposed. Under the optimized experimental conditions, the enhanced fluorescence intensity ratio (DeltaF/F(0)) was linear with the concentration of glucose (c) in the range of 0.0-180 microg ml(-1) with a detection limit of 0.8 microg ml(-1) (S/N=3). The R.S.D. values were 0.33% and 0.37% at the levels of 22.5 and 100 microg ml(-1), respectively. The proposed method was applied to the determination of glucose in synthetic samples with satisfactory results. The proposed method was also performed to the analysis of blood glucose in human serum samples and the results were in good agreement with clinical data provided by the hospital, which indicates that the method presented here is not only simple, sensitive, but also reliable and suitable for practical applications.
成功地使用改进的 Stöber 法合成了氨基功能化的掺 Eu(TTA)(3)phen 的荧光硅纳米粒子 (LSNPs)，Eu(TTA)(3)phen 是 Eu(III)的混合配合物，其中 TTA 是 2-噻吩甲酰三氟丙酮，phen 是 1,10-邻菲罗啉。通过扫描电子显微镜 (SEM)、透射电子显微镜 (TEM)、傅里叶变换红外 (FTIR) 和荧光光谱对合成的纳米粒子进行了表征。在葡萄糖存在下，由于荧光共振能量转移的增强，氨基功能化 LSNPs 的荧光强度增强。基于荧光增强效应，提出了一种用于测定葡萄糖的简单灵敏方法。在优化的实验条件下，增强荧光强度比 (DeltaF/F(0)) 与葡萄糖浓度 (c) 在 0.0-180 μg ml(-1) 范围内呈线性关系，检测限为 0.8 μg ml(-1) (S/N=3)。在 22.5 和 100 μg ml(-1) 的水平下，R.S.D. 值分别为 0.33%和 0.37%。该方法用于测定合成样品中的葡萄糖，结果令人满意。该方法还用于人血清样品中血糖的分析，结果与医院提供的临床数据吻合良好，表明该方法不仅简单、灵敏，而且可靠，适用于实际应用。
