Rusin P, Markiewicz L, Olszewski J, Morawiec-Sztandera A, Kowalski M, Przybylowska K, Kaczmarczyk D, Kusmierczyk K, Majsterek I
Department of Molecular Genetics, University of Lodz, Lodz, Poland.
Exp Oncol. 2009 Sep;31(3):168-73.
To evaluate the generation and repair of DNA double strand breaks (DSBs) as a critical factors that define the efficiency of radiation therapy of cancer patients.
Peripheral blood lymphocytes obtained from 18 patients with head and neck squamous cell carcinoma (HNSCC) and 18 healthy donors were studied. The efficiency of DSBs repair after genotoxic treatment with hydrogen peroxide and gamma-radiation were examined by neutral comet assay. MTT assay was used for cell viability analysis and Annexin V-FITC kit specific for kinase-3 was employed to determine apoptosis.
Lymphocytes from HNSCC patients were sensitive to genotoxic treatment and displayed impaired DSBs repair. Finally, as a consequence of this finding we have evidenced higher rate of apoptosis induction after gamma-radiation treatment of lymphocytes from HNSCC patients than those from healthy controls.
DSBs repair and increased apoptosis in cells of patients with head and neck cancer is relevant for efficient therapy of HNSCC.
评估DNA双链断裂（DSB）的产生和修复，这是决定癌症患者放射治疗效果的关键因素。
研究了从18例头颈部鳞状细胞癌（HNSCC）患者和18名健康供体获得的外周血淋巴细胞。用过氧化氢和γ射线进行基因毒性处理后，通过中性彗星试验检测DSB修复效率。采用MTT法进行细胞活力分析，并使用针对激酶-3的膜联蛋白V-FITC试剂盒测定细胞凋亡。
HNSCC患者的淋巴细胞对基因毒性处理敏感，DSB修复受损。最后，基于这一发现，我们证明了HNSCC患者淋巴细胞经γ射线照射后诱导凋亡的速率高于健康对照者。
头颈部癌症患者细胞中的DSB修复和凋亡增加与HNSCC的有效治疗相关。
