用于测定生物传感器的聚合物膜离子选择性电极的电流驱动离子通量。

Current-driven ion fluxes of polymeric membrane ion-selective electrode for potentiometric biosensing.

机构信息

Key Laboratory of Coastal Environmental Processes, Yantai Institute of Coastal Zone Research for Sustainable Development, Chinese Academy of Sciences, Yantai 264003, PR China.

出版信息

J Am Chem Soc. 2009 Oct 21;131(41):14640-1. doi: 10.1021/ja906723h.

DOI:10.1021/ja906723h

PMID:19785410

Abstract

A polymeric membrane ion-selective electrode (ISE) for rapid potentiometric biosensing has been developed. Butyrylcholinesterase, as a target enzyme, is immobilized on the surface of the polymeric membrane. A macro-command-controlled procedure for switching between the potentiostatic and galvanostatic steps has been designed to provide a current-driven release of the butyrylcholine substrate for in situ biosensing of the enzyme and its inhibitors. This system has the flexibility of trapping substrate ions in the ISE inner filling solution and provides rapid, continuous, and reproducible measurements of enzymes and other bioanalytes involved in enzymatic systems.

摘要

已开发出一种用于快速电位生物传感的聚合物膜离子选择性电极（ISE）。丁酰胆碱酯酶作为靶酶固定在聚合物膜的表面上。设计了一种宏观命令控制程序，用于在恒电位和恒电流步骤之间切换，以提供电流驱动的丁酰胆碱底物释放，用于酶及其抑制剂的原位生物传感。该系统具有在 ISE 内填充溶液中捕获底物离子的灵活性，并提供涉及酶系统的酶和其他生物分析物的快速、连续和可重复的测量。