准分子激光原位角膜磨镶术后丝裂霉素C在房水中的浓度：一项实验研究。

Mitomycin C aqueous humor concentration after photorefractive keratectomy: an experimental study.

作者信息

Kymionis George D, Diakonis Vasilios F, Panagopoulou Sophia I, Grentzelos Michael A, Kazakos Dimitris C, Tzatzarakis Manolis N, Tsatsakis Aristidis M, Pallikaris Aristophanis I

机构信息

Institute of Vision and Optics, Department of Medicine, University of Crete, Medical School, Heraklion, Crete - Greece.

出版信息

Eur J Ophthalmol. 2009 Sep-Oct;19(5):738-42. doi: 10.1177/112067210901900509.

DOI:10.1177/112067210901900509

PMID:19787591

Abstract

PURPOSE

To evaluate mitomycin C (MMC) aqueous humor concentration after photorefractive keratectomy (PRK).

METHODS

In this experimental study, twenty-four eyes of 12 male pigmented rabbits were divided into 4 groups and studied at the Institute of Vision and Optics, Department of Medicine, University of Crete, Greece. Eyes in groups 1 and 2 underwent PRK to correct -5 diopters (D) in a 6-mm optical zone, while sponges soaked with 0.02% MMC were applied on the exposed corneal stroma for 60 and 120 seconds, respectively. Similarly, eyes in groups 3 and 4 underwent PRK to correct -10 D in a 6-mm optical zone, while sponges soaked with 0.02% MMC were applied on the exposed corneal stroma for 60 and 120 seconds, respectively. Aqueous humor was extracted from all rabbit eyes 10 minutes after MMC application and high-performance liquid chromatography was performed immediately to detect and quantify MMC levels.

RESULTS

The mean aqueous humor concentration of MMC was 0.23+/-0.03 microg/mL, 0.39+/-0.05 microg/mL, 0.28+/-0.04 microg/mL, and 0.52+/-0.16 microg/mL in groups 1, 2, 3, and 4, respectively. The effect of application time and correction on aqueous humor MMC concentration was significant (p<0.0001 and p=0.019), while the exposure time had a greater impact on aqueous humor MMC concentration when compared with the attempted correction.

CONCLUSIONS

Both exposure time of MMC on the corneal stroma and the attempted correction was correlated with MMC aqueous humor concentrations.

摘要

目的

评估准分子激光角膜切削术（PRK）后丝裂霉素C（MMC）的房水浓度。

方法

在这项实验研究中，12只雄性有色家兔的24只眼睛被分为4组，在希腊克里特大学医学院视觉与光学研究所进行研究。第1组和第2组的眼睛接受PRK以矫正6毫米光学区的-5屈光度（D），同时将浸泡有0.02%MMC的海绵分别应用于暴露的角膜基质60秒和120秒。同样，第3组和第4组的眼睛接受PRK以矫正6毫米光学区的-10 D，同时将浸泡有0.02%MMC的海绵分别应用于暴露的角膜基质60秒和120秒。在应用MMC后10分钟从所有兔眼中提取房水，并立即进行高效液相色谱法以检测和定量MMC水平。

结果

第1、2、3和4组中MMC的平均房水浓度分别为0.23±0.03微克/毫升、0.39±0.05微克/毫升、0.28±0.04微克/毫升和0.52±0.16微克/毫升。应用时间和矫正对房水MMC浓度的影响具有显著性（p<0.0001和p=0.019），而与尝试矫正相比，暴露时间对房水MMC浓度的影响更大。