Hougen H P, Jensen E T
University Institute of Forensic Medicine, Rigshospitalet, Copenhagen, Denmark.
APMIS. 1990 Nov;98(11):1015-21.
The protective effect of primed lymphocytes against a lethal dose of Salmonella typhimurium was studied in athymic and euthymic LEW rats. Primed lymphocytes were obtained by inoculating euthymic and thymus-grafted animals with a non-lethal dose of Salmonella typhimurium. Four weeks after the infection, spleen lymphocytes were separated by panning technique and antibody-coated magnetic microsphere separation using antibodies to pan T and pan B lymphocytes and subsequent sorting in a fluorescence activated cell sorter by means of monoclonal antibodies against CD4+ and CD8+ cells. Euthymic and athymic rats were injected with different doses of primed pan B, pan T, CD4+ and CD8+ T lymphocytes before inoculation with a lethal bacterial dose. Most of the animals treated with pan B, pan T or CD8+ cells died within two weeks. After treatment with primed CD4+ cells, only six of 39 animals died. Doses as low as 10(4) cells from both euthymic or thymus-grafted animals were effective, and athymic and euthymic recipients survived equally well. Four weeks after the infection both athymic and euthymic animals housed very few bacteria and had high antibacterial antibody titres. The percentages of splenic and lymph node CD4+ cells in the athymic rats were comparable to those found in the euthymic animals. The study shows that primed CD4+ lymphocytes even in very low doses are able to induce immunity against a Salmonella typhimurium infection.
在无胸腺和有胸腺的LEW大鼠中研究了致敏淋巴细胞对致死剂量鼠伤寒沙门氏菌的保护作用。通过用非致死剂量的鼠伤寒沙门氏菌接种有胸腺和胸腺移植的动物来获得致敏淋巴细胞。感染四周后，通过淘选技术以及使用针对全T和全B淋巴细胞的抗体进行抗体包被磁微球分离，随后借助针对CD4 +和CD8 +细胞的单克隆抗体在荧光激活细胞分选仪中进行分选，分离出脾淋巴细胞。在接种致死剂量细菌之前，给有胸腺和无胸腺的大鼠注射不同剂量的致敏全B、全T、CD4 +和CD8 + T淋巴细胞。大多数接受全B、全T或CD8 +细胞治疗的动物在两周内死亡。用致敏CD4 +细胞治疗后，39只动物中只有6只死亡。来自有胸腺或胸腺移植动物的低至10(4)个细胞的剂量都是有效的，无胸腺和有胸腺的受体存活情况同样良好。感染四周后，无胸腺和有胸腺的动物体内细菌数量都很少，并且具有高抗菌抗体滴度。无胸腺大鼠脾脏和淋巴结中CD4 +细胞的百分比与有胸腺动物中的相当。该研究表明，即使是非常低剂量的致敏CD4 +淋巴细胞也能够诱导针对鼠伤寒沙门氏菌感染的免疫力。
