Hojo Kazuhiro, Hakamata Hideki, Takahashi Aya, Hosokawa Yu-Ya, Kusu Fumiyo
Department of Analytical Chemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Horinouchi 1432-1, Hachioji, Tokyo 192-0392, Japan.
Biomed Chromatogr. 2010 Jun;24(6):600-5. doi: 10.1002/bmc.1332.
A simple and sensitive method that dose not require derivatization for determining cholestanol has been developed using HPLC with electrochemical detection (HPLC-ECD). The current peak height was linearly related to the amount of cholestanol injected, ranging from 1 to 200 muM (r = 0.999). The detection limit (S/N = 3) of cholestanol was 0.23 muM (1.2 pmol). Total cholestanol in control human and mouse serum was determined by the present method with a recovery rate of more than 90% and an RSD (n = 5) of less than 7.3%. Further, this method was successfully applied to monitor experimental hypercholestanolemia in mice fed a high-cholestanol diet, an animal model of cerebrotendinous xanthomatosis (CTX). In conclusion, we found this method to be both simple and useful for the determination of cholestanol in serum, helping in the diagnosis of CTX.
已开发出一种简单且灵敏的方法，使用高效液相色谱 - 电化学检测法（HPLC - ECD）测定胆甾烷醇，该方法无需衍生化。电流峰高与注入的胆甾烷醇量呈线性关系，范围为1至200 μM（r = 0.999）。胆甾烷醇的检测限（S/N = 3）为0.23 μM（1.2 pmol）。用本方法测定对照人血清和小鼠血清中的总胆甾烷醇，回收率超过90%，相对标准偏差（n = 5）小于7.3%。此外，该方法成功应用于监测喂食高胆甾烷醇饮食的小鼠实验性高胆甾烷醇血症，这是脑腱黄瘤病（CTX）的动物模型。总之，我们发现该方法对于血清中胆甾烷醇的测定既简单又有用，有助于CTX的诊断。
