Yi Yong-Yan, Li De-Rong, Zhang Yun-Wei, Yang Fu-Yu
Grassland Science Department of Jiangxi Agricultural University, Nanchang 330045, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2009 Jul;29(7):1826-9.
The invasion extent and harmfulness of fungi can be determined by chitin, ergosterol and mycotoxins. It is important to monitor chitin, ergosterol and mycotoxins changes to prevent contamination of forage and feed products, and effectively control the sustainable development of the mildew. Predication of these chemical materials was often completed by laboratory analysis, which was time-consuming and cumbersome and could not reflect the results in time in the past. Near infrared reflectance spectroscopy (NIRS) is a rapid, convenient, highly efficient, nondestructive and low-cost analytical technique, which has been widely used in various fields such as food field and feed field for quantitative and qualitative analysis. It has a great potentiality of application in quality analysis. In this paper, the principle and the characteristic of NIRS and its applications in food, forage, feed and other agriculture products quality analysis were introduced. Its applications in fungal biomass (chitin, ergosterol) and mycotoxins were mainly reviewed. NIRS was used to quantify chitin, ergosterol and mycotoxins. Calibration equations and validation equations for these materials were developed. It is also expected that NIRS will play a more and more important role in the field of fungi with the establishment of calibration equation and improvement of model database.
几丁质、麦角固醇和霉菌毒素可用于确定真菌的侵染程度和危害程度。监测几丁质、麦角固醇和霉菌毒素的变化对于防止草料和饲料产品受到污染以及有效控制霉菌的可持续发展至关重要。过去,这些化学物质的预测通常通过实验室分析来完成,这种方法既耗时又繁琐,而且无法及时反映结果。近红外反射光谱法(NIRS)是一种快速、便捷、高效、无损且低成本的分析技术,已广泛应用于食品领域和饲料领域等各个领域的定量和定性分析。它在质量分析方面具有很大的应用潜力。本文介绍了近红外反射光谱法的原理、特点及其在食品、草料、饲料和其他农产品质量分析中的应用。主要综述了其在真菌生物量(几丁质、麦角固醇)和霉菌毒素方面的应用。利用近红外反射光谱法定量分析几丁质、麦角固醇和霉菌毒素。建立了这些物质的校准方程和验证方程。随着校准方程的建立和模型数据库的完善,预计近红外反射光谱法在真菌领域将发挥越来越重要的作用。