Wang Yan-Jun, Yao Hui-Lu, Wang Gui-Wen, Wang Yun, Feng Mei-Fu
State Key Lab of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2009 Jul;29(7):1881-3.
Single cell laser tweezers Raman spectroscopy (LTRS) has been applied to biology field. In the present article, the authors measured the spectra of liver cancer cells, para-cancer cells and normal hepatocytes using single cell laser tweezer Raman spectroscopy (LTRS) system and compared their average spectra changes. The results showed that the laser tweezers Raman spectroscopy could differentiate specimens of different pathological changes from liver tissue studied. The 1 070 and 1 266 cm(-1) peaks obtained from normal hepatocytes were more visible than the same two peaks obtained from liver cancer and para-cancer specimen. The 1 445 cm(-1) peak of normal hepatocytes was higher than that of liver cancer cells and para-cancer cells. It is known that the 1 070 cm(-1) peak represents lipids and nucleic acids, while 1 266 and 1 445 cm(-1) peaks represent lipids and proteins. So, these peak changes may directly reflect the changed biomaterials related to liver carcinogenesis. Thus, single cell laser tweezer Raman spectroscopy may be a nondestructive, rapid and good method to measure and analyze different pathological specimens from liver cancer.
单细胞激光镊子拉曼光谱（LTRS）已应用于生物学领域。在本文中，作者使用单细胞激光镊子拉曼光谱（LTRS）系统测量了肝癌细胞、癌旁细胞和正常肝细胞的光谱，并比较了它们的平均光谱变化。结果表明，激光镊子拉曼光谱能够区分所研究肝脏组织中不同病理变化的标本。正常肝细胞获得的1070和1266 cm⁻¹峰比肝癌和癌旁标本获得的相同两个峰更明显。正常肝细胞的1445 cm⁻¹峰高于肝癌细胞和癌旁细胞。已知1070 cm⁻¹峰代表脂质和核酸，而1266和1445 cm⁻¹峰代表脂质和蛋白质。因此，这些峰的变化可能直接反映与肝癌发生相关的生物材料的变化。因此，单细胞激光镊子拉曼光谱可能是一种用于测量和分析肝癌不同病理标本的无损、快速且良好的方法。
