Xi Gang, Yang Yun-Jing, Lu Hong
Department of Physics, Xi'an University of Technology, Xi'an 710048, China
Guang Pu Xue Yu Guang Pu Fen Xi. 2009 Jul;29(7):1920-4.
A system for studying biological effect of radio frequency electromagnetic field was developed. The system can form an area where electromagnetic wave with large frequency range is well distributed. The strength of electromagnetic wave was measured easily. Electromagnetic wave in the system did not have effect on environment. The sensitivity of spinach chloroplast membrane to low intensity electromagnetic radiation of 300 MHz under power density of 5 mW x cm(-2) was studied by the spectral analysis method of fluorescence of 8-anilino-1-naphthalene-sulfonic acid (ANS) and the changes in chlorophyll a (Chla) fluorescence parameters of spinach chloroplast membrane. The result showed that the position of spectrum of ANS fluorescence of spinach chloroplast membrane did not change, but the intensity of ANS fluorescence was obviously increased under the action of electromagnetic radiation with power density of 1-5 mW x cm(-2). There was an increase in the intensity of ANS fluorescence with the increase in electromagnetic radiation. The increase of ANS fluorescence of spinach chloroplast membrane showed that low level electromagnetic field induced the decrease in fluidity of chloroplast membrane compared with control experiment. The cause of the change in the fluidity could be related to the polarization of chloroplast membrane under the electromagnetic field. The analysis of Chla fluorescence parameters of spinach chloroplast membrane indicated that low level electromagnetic field of 300 MHz made the fluorescence parameters F0 and F(VI/)F(V) decrease, and F(V)/Fo, Fv/F(m) and deltaF(V)/T increase. It was showed that low level electromagnetic field caused the change of non-active center of photosystem II of spinach chloroplast membrane to active center and the increase in potential active and photochemical efficiency of PSII, and promoted the transmit process of electron in photosynthesis of chloroplast membrane of photosynthesis cell in spinach leaf. The study confirmed that low level electromagnetic field has non--thermal effects on photosynthesis system of spinach chloroplast membrane. The cell in spinach leaf can keep the photosynthesis through the change in heterogeneity of photosystem II and adapt to the environment of electromagnetic radiation increase.
研制了一种用于研究射频电磁场生物效应的系统。该系统能够形成一个大频率范围的电磁波分布良好的区域。电磁波强度易于测量。系统中的电磁波对环境没有影响。采用8-苯胺基-1-萘磺酸（ANS）荧光光谱分析法以及菠菜叶绿体膜叶绿素a（Chla）荧光参数的变化，研究了在功率密度为5 mW·cm⁻² 下菠菜叶绿体膜对300 MHz低强度电磁辐射的敏感性。结果表明，菠菜叶绿体膜ANS荧光光谱位置未变，但在功率密度为1 - 5 mW·cm⁻² 的电磁辐射作用下，ANS荧光强度明显增加。ANS荧光强度随电磁辐射增强而增加。菠菜叶绿体膜ANS荧光增加表明，与对照实验相比，低水平电磁场导致叶绿体膜流动性降低。流动性变化的原因可能与电磁场作用下叶绿体膜的极化有关。菠菜叶绿体膜Chla荧光参数分析表明，300 MHz低水平电磁场使荧光参数F0和F(VI/)F(V)降低，F(V)/Fo、Fv/F(m)和deltaF(V)/T增加。结果表明，低水平电磁场使菠菜叶绿体膜光系统II的非活性中心向活性中心转变，PSII潜在活性和光化学效率增加，促进了菠菜叶片光合作用细胞叶绿体膜光合作用中电子的传递过程。该研究证实低水平电磁场对菠菜叶绿体膜光合作用系统具有非热效应。菠菜叶片中的细胞可通过光系统II异质性的变化维持光合作用，并适应电磁辐射增强的环境。
