[在原核生物中表达重组蛋白程序性死亡受体配体1（PD-L1）并分析其生物学活性]
[Express the recombinant protein PD-L1 in prokaryotic and analyze its biological activity].
作者信息
Shen Li-Ping, Chen Si-Yong, Bian Tao, Yi Yao, Wang Feng, Qiu Feng, Bi Sheng-Li
机构信息
National Institute of Viral Disease Prevention and Control, Chinese CDC , Beijing 100052, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2009 Feb;23(1):5-7.
OBJECTIVE
To construct the programmed cell death 1 ligant 1 (PD-L1) recombination expression vector, express the fusion protein in prokaryotic and analyze the biological action of express product.
METHODS
The whole PD-L1 gene sequence was synthesized after codon optimized. Construct the thioredoxin-(PD-L1) recombination expression vector and express the fusion protein in E. coli. Purified the target protein and analyze the conjugated ability of protein by ELISA.
RESULTS
The PD-L1 recombinant expression vector has been constructed correctly. The target protein has been obtained with which expressed in high efficiency and production. The target protein can conjugate specifically with the PD-1, its specific receptor.
CONCLUSION
We have obtained the PD-L1 recombinant protein success with high biological activity. The result provide the basic condition for further study on antibody and mutually action between PD-L1 and chronic virus infectious.
目的
构建程序性细胞死亡蛋白1配体1（PD-L1）重组表达载体，在原核细胞中表达融合蛋白并分析表达产物的生物学作用。
方法
对PD-L1基因序列进行密码子优化后全序列合成。构建硫氧还蛋白-（PD-L1）重组表达载体并在大肠杆菌中表达融合蛋白。纯化目的蛋白并用ELISA分析蛋白结合能力。
结果
正确构建了PD-L1重组表达载体。获得了高效表达且产量高的目的蛋白。目的蛋白能与其特异性受体PD-1特异性结合。
结论
成功获得了具有高生物学活性的PD-L1重组蛋白。该结果为进一步研究PD-L1抗体及与慢性病毒感染的相互作用提供了基础条件。
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