通过代谢工程改造三羧酸循环以提高谷氨酸棒杆菌产赖氨酸。
Metabolic engineering of the tricarboxylic acid cycle for improved lysine production by Corynebacterium glutamicum.
机构信息
Institute of Biochemical Engineering, Technische Universität Braunschweig, Gaussstr. 17, D-38106 Braunschweig, Germany.
出版信息
Appl Environ Microbiol. 2009 Dec;75(24):7866-9. doi: 10.1128/AEM.01942-09. Epub 2009 Oct 9.
Abstract
In the present work, lysine production by Corynebacterium glutamicum was improved by metabolic engineering of the tricarboxylic acid (TCA) cycle. The 70% decreased activity of isocitrate dehydrogenase, achieved by start codon exchange, resulted in a >40% improved lysine production. By flux analysis, this could be correlated to a flux shift from the TCA cycle toward anaplerotic carboxylation.
摘要
在本工作中，通过代谢工程改造三羧酸（TCA）循环提高了谷氨酸棒杆菌的赖氨酸产量。通过起始密码子替换使异柠檬酸脱氢酶的活性降低了 70%，导致赖氨酸产量提高了超过 40%。通过通量分析，这可以与从 TCA 循环向补料羧化的通量转移相关联。
相似文献
1
Metabolic engineering of the tricarboxylic acid cycle for improved lysine production by Corynebacterium glutamicum.通过代谢工程改造三羧酸循环以提高谷氨酸棒杆菌产赖氨酸。
Appl Environ Microbiol. 2009 Dec;75(24):7866-9. doi: 10.1128/AEM.01942-09. Epub 2009 Oct 9.
2
Rational modification of tricarboxylic acid cycle for improving L-lysine production in Corynebacterium glutamicum.理性改造三羧酸循环以提高谷氨酸棒杆菌产赖氨酸。
Microb Cell Fact. 2018 Jul 7;17(1):105. doi: 10.1186/s12934-018-0958-z.
3
In-depth profiling of lysine-producing Corynebacterium glutamicum by combined analysis of the transcriptome, metabolome, and fluxome.通过转录组、代谢组和通量组联合分析对产赖氨酸谷氨酸棒杆菌进行深度剖析。
J Bacteriol. 2004 Mar;186(6):1769-84. doi: 10.1128/JB.186.6.1769-1784.2004.
4
Metabolic fluxes in Corynebacterium glutamicum during lysine production with sucrose as carbon source.以蔗糖为碳源生产赖氨酸时谷氨酸棒杆菌中的代谢通量
Appl Environ Microbiol. 2004 Dec;70(12):7277-87. doi: 10.1128/AEM.70.12.7277-7287.2004.
5
Increased lysine production by flux coupling of the tricarboxylic acid cycle and the lysine biosynthetic pathway--metabolic engineering of the availability of succinyl-CoA in Corynebacterium glutamicum.通过三羧酸循环和赖氨酸生物合成途径的通量偶联增加赖氨酸产量——谷氨酸棒杆菌中琥珀酰辅酶 A 可用性的代谢工程。
Metab Eng. 2013 Jan;15:184-95. doi: 10.1016/j.ymben.2012.07.005. Epub 2012 Aug 2.
6
Systems-wide metabolic pathway engineering in Corynebacterium glutamicum for bio-based production of diaminopentane.在谷氨酸棒杆菌中进行全系统代谢途径工程以生物合成二氨基戊烷。
Metab Eng. 2010 Jul;12(4):341-51. doi: 10.1016/j.ymben.2010.03.005. Epub 2010 Apr 8.
7
Exploring lysine riboswitch for metabolic flux control and improvement of L-lysine synthesis in Corynebacterium glutamicum.探索赖氨酸核糖开关以控制代谢通量并提高谷氨酸棒杆菌中L-赖氨酸的合成
ACS Synth Biol. 2015 Jun 19;4(6):729-34. doi: 10.1021/sb500332c. Epub 2015 Jan 16.
8
Next-generation sequencing-based transcriptome analysis of L-lysine-producing Corynebacterium glutamicum ATCC 21300 strain.基于高通量测序的产赖氨酸谷氨酸棒杆菌 ATCC 21300 菌株转录组分析。
J Microbiol. 2013 Dec;51(6):877-80. doi: 10.1007/s12275-013-3236-0. Epub 2013 Dec 19.
9
Carbohydrate metabolism in Corynebacterium glutamicum and applications for the metabolic engineering of L-lysine production strains.谷氨酸棒杆菌中的碳水化合物代谢及其在 L-赖氨酸生产菌株代谢工程中的应用。
Appl Microbiol Biotechnol. 2010 May;86(5):1313-22. doi: 10.1007/s00253-010-2537-z. Epub 2010 Mar 24.
10
Attenuating l-lysine production by deletion of ddh and lysE and their effect on l-threonine and l-isoleucine production in Corynebacterium glutamicum.通过缺失ddh和lysE减弱谷氨酸棒杆菌中L-赖氨酸的产生及其对L-苏氨酸和L-异亮氨酸产生的影响。
Enzyme Microb Technol. 2016 Nov;93-94:70-78. doi: 10.1016/j.enzmictec.2016.07.013. Epub 2016 Jul 25.
引用本文的文献
1
Metabolic engineering strategies for L-Homoserine production in Escherichia coli.大肠杆菌中L-高丝氨酸生产的代谢工程策略。
Microb Cell Fact. 2024 Dec 19;23(1):338. doi: 10.1186/s12934-024-02623-7.
2
Recent Advances in Metabolic Engineering for the Biosynthesis of Phosphoenol Pyruvate-Oxaloacetate-Pyruvate-Derived Amino Acids.磷酸烯醇丙酮酸-草酰乙酸-丙酮酸衍生氨基酸生物合成的代谢工程最新进展。
Molecules. 2024 Jun 18;29(12):2893. doi: 10.3390/molecules29122893.
3
Developing a High-Umami, Low-Salt Soy Sauce through Accelerated Moromi Fermentation with and Strains.通过使用[具体菌株1]和[具体菌株2]进行速酿发酵来开发高鲜味、低盐酱油。
Foods. 2024 Apr 30;13(9):1386. doi: 10.3390/foods13091386.
4
Systems biology of industrial oxytetracycline production in Streptomyces rimosus: the secrets of a mutagenized hyperproducer.工业生产土霉素的里氏木霉系统生物学：诱变高产菌的秘密。
Microb Cell Fact. 2023 Oct 28;22(1):222. doi: 10.1186/s12934-023-02215-x.
5
Integrative transcriptome and proteome revealed high-yielding mechanisms of epsilon-poly-L-lysine by .整合转录组和蛋白质组揭示了ε-聚-L-赖氨酸的高产机制 。
你提供的原文似乎不完整，“by”后面缺少具体内容。
Front Microbiol. 2023 Apr 20;14:1123050. doi: 10.3389/fmicb.2023.1123050. eCollection 2023.
6
High-Quality Genome-Scale Reconstruction of ATCC 13032.ATCC 13032的高质量基因组规模重建
Front Microbiol. 2021 Nov 15;12:750206. doi: 10.3389/fmicb.2021.750206. eCollection 2021.
7
Recent progress in production of amino acid-derived chemicals using Corynebacterium glutamicum.利用谷氨酸棒杆菌生产氨基酸衍生化学品的最新进展。
World J Microbiol Biotechnol. 2021 Feb 11;37(3):49. doi: 10.1007/s11274-021-03007-4.
8
A common approach for absolute quantification of short chain CoA thioesters in prokaryotic and eukaryotic microbes.一种用于原核和真核微生物中短链 CoA 硫酯绝对定量的常用方法。
Microb Cell Fact. 2020 Aug 10;19(1):160. doi: 10.1186/s12934-020-01413-1.
9
Equilibrium of the intracellular redox state for improving cell growth and L-lysine yield of Corynebacterium glutamicum by optimal cofactor swapping.通过最优辅因子替换实现细胞内氧化还原状态平衡，以提高谷氨酸棒杆菌的细胞生长和 L-赖氨酸产量。
Microb Cell Fact. 2019 Apr 3;18(1):65. doi: 10.1186/s12934-019-1114-0.
10
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) co-produced with L-isoleucine in Corynebacterium glutamicum WM001.在谷氨酸棒杆菌 WM001 中与 L-异亮氨酸共生产聚（3-羟基丁酸-co-3-羟基戊酸）。
Microb Cell Fact. 2018 Jun 15;17(1):93. doi: 10.1186/s12934-018-0942-7.
本文引用的文献
1
In silico genome-scale reconstruction and validation of the Corynebacterium glutamicum metabolic network.谷氨酸棒杆菌代谢网络的计算机基因组规模重建与验证
Biotechnol Bioeng. 2009 Feb 1;102(2):583-97. doi: 10.1002/bit.22067.
2
Metabolic responses to pyruvate kinase deletion in lysine producing Corynebacterium glutamicum.赖氨酸产生短杆菌谷氨酸代谢对丙酮酸激酶缺失的响应。
Microb Cell Fact. 2008 Mar 13;7:8. doi: 10.1186/1475-2859-7-8.
3
Metabolic flux engineering of L-lysine production in Corynebacterium glutamicum--over expression and modification of G6P dehydrogenase.谷氨酸棒杆菌中L-赖氨酸生产的代谢通量工程——葡萄糖-6-磷酸脱氢酶的过表达与修饰
J Biotechnol. 2007 Oct 31;132(2):99-109. doi: 10.1016/j.jbiotec.2007.05.026. Epub 2007 Jun 6.
4
Amplified expression of fructose 1,6-bisphosphatase in Corynebacterium glutamicum increases in vivo flux through the pentose phosphate pathway and lysine production on different carbon sources.谷氨酸棒杆菌中果糖1,6 -二磷酸酶的过表达增加了体内戊糖磷酸途径的通量以及在不同碳源上的赖氨酸产量。
Appl Environ Microbiol. 2005 Dec;71(12):8587-96. doi: 10.1128/AEM.71.12.8587-8596.2005.
5
Systems biotechnology for strain improvement.用于菌株改良的系统生物技术。
Trends Biotechnol. 2005 Jul;23(7):349-58. doi: 10.1016/j.tibtech.2005.05.003.
6
In vivo quantification of intracellular amino acids and intermediates of the methionine pathway in Corynebacterium glutamicum.谷氨酸棒杆菌细胞内氨基酸及甲硫氨酸途径中间产物的体内定量分析。
Anal Biochem. 2005 May 1;340(1):171-3. doi: 10.1016/j.ab.2005.01.027.
7
A novel gnd mutation leading to increased L-lysine production in Corynebacterium glutamicum.一种导致谷氨酸棒杆菌中L-赖氨酸产量增加的新型gnd突变。
FEMS Microbiol Lett. 2005 Jan 15;242(2):265-74. doi: 10.1016/j.femsle.2004.11.014.
8
In-depth profiling of lysine-producing Corynebacterium glutamicum by combined analysis of the transcriptome, metabolome, and fluxome.通过转录组、代谢组和通量组联合分析对产赖氨酸谷氨酸棒杆菌进行深度剖析。
J Bacteriol. 2004 Mar;186(6):1769-84. doi: 10.1128/JB.186.6.1769-1784.2004.
9
Comparative metabolic flux analysis of lysine-producing Corynebacterium glutamicum cultured on glucose or fructose.在葡萄糖或果糖上培养的产赖氨酸谷氨酸棒杆菌的比较代谢通量分析。
Appl Environ Microbiol. 2004 Jan;70(1):229-39. doi: 10.1128/AEM.70.1.229-239.2004.
10
Identification and characterization of the last two unknown genes, dapC and dapF, in the succinylase branch of the L-lysine biosynthesis of Corynebacterium glutamicum.谷氨酸棒杆菌L-赖氨酸生物合成的琥珀酰化分支中最后两个未知基因dapC和dapF的鉴定与表征。
J Biotechnol. 2003 Sep 4;104(1-3):199-211. doi: 10.1016/s0168-1656(03)00156-1.
Zotero 插件