Hong Hao, Dong Nianguo, Shi Jiawei, Chen Si, Guo Chao, Hu Ping, Qi Hongxu
Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
J Huazhong Univ Sci Technolog Med Sci. 2009 Oct;29(5):599-603. doi: 10.1007/s11596-009-0513-6. Epub 2009 Oct 11.
The aim of this study was to fabricate biomatrix/polymer hybrid scaffolds using an electrospinning technique. Then tissue engineered heart valves were engineered by seeding mesenchymal stromal cells (MSCs) onto the scaffolds. The effects of the hybrid scaffolds on the proliferation of seed cells, formation of extracellular matrix and mechanical properties of tissue engineered heart valves were investigated. MSCs were obtained from rats. Porcine aortic heart valves were decellularized, coated with poly(3-hydroxybutyrate-co-4-hydroxybutyrate) using an electrospinning technique, and reseeded and cultured over a time period of 14 days. In control group, the decellularized valve scaffolds were reseeded and cultured over an equivalent time period. Specimens of each group were examined histologically (hematoxylin-eosin [HE] staining, immunohistostaining, and scanning electron microscopy), biochemically (DNA and 4-hydroxyproline) and mechanically. The results showed that recellularization was comparable to the specimens of hybrid scaffolds and controls. The specimens of hybrid scaffolds and controls revealed comparable amounts of cell mass and 4-hydroxyproline (P>0.05). However, the specimens of hybrid scaffolds showed a significant increase in mechanical strength, compared to the controls (P<0.05). This study demonstrated the superiority of the hybrid scaffolds to increase the mechanical strength of tissue engineered heart valves. And compared to the decellularized valve scaffolds, the hybrid scaffolds showed similar effects on the proliferation of MSCs and formation of extracellular matrix. It was believed that the hybrid scaffolds could be used for the construction of tissue engineered heart valves.
本研究的目的是使用静电纺丝技术制备生物基质/聚合物混合支架。然后通过将间充质基质细胞（MSCs）接种到支架上来构建组织工程心脏瓣膜。研究了混合支架对种子细胞增殖、细胞外基质形成以及组织工程心脏瓣膜力学性能的影响。MSCs取自大鼠。将猪主动脉心脏瓣膜去细胞化，采用静电纺丝技术用聚（3-羟基丁酸酯-共-4-羟基丁酸酯）包被，重新接种并培养14天。在对照组中，将去细胞化的瓣膜支架重新接种并在相同时间段内培养。对每组标本进行组织学检查（苏木精-伊红[HE]染色、免疫组织化学染色和扫描电子显微镜检查）、生化检查（DNA和4-羟基脯氨酸）以及力学检查。结果表明，再细胞化与混合支架和对照组的标本相当。混合支架和对照组的标本显示出相当的细胞量和4-羟基脯氨酸含量（P>0.05）。然而，与对照组相比，混合支架的标本在机械强度上有显著增加（P<0.05）。本研究证明了混合支架在提高组织工程心脏瓣膜机械强度方面的优越性。并且与去细胞化瓣膜支架相比，混合支架对MSCs增殖和细胞外基质形成的影响相似。据信，混合支架可用于构建组织工程心脏瓣膜。
