酵母中的油酸β-氧化涉及硫酯酶，但不涉及不是二烯酰辅酶A异构酶的Yor180c蛋白。
Oleate beta-oxidation in yeast involves thioesterase but not Yor180c protein that is not a dienoyl-CoA isomerase.
作者信息
Ntamack André G, Karpichev Igor V, Gould Stephen J, Small Gillian M, Schulz Horst
机构信息
Department of Chemistry, City College and Graduate School of the City University of New York, New York, NY 10031, USA.
出版信息
Biochim Biophys Acta. 2009 May;1791(5):371-8. doi: 10.1016/j.bbalip.2009.01.026.
The beta-oxidation of oleic acid in Saccharomyces cerevisiae (S. cerevisiae) was studied by comparing the growth of wild-type cells on oleic acid or palmitic acid with the growth of mutants that either had a deletion in the YOR180c (DCI1) gene reported to encode delta3,5,delta2,4-dienoyl-CoA isomerase (dienoyl-CoA isomerase) or in the PTE1 gene encoding peroxisomal thioesterase 1. Growth of wild-type cells was indistinguishable from that of YOR180c mutant cells on either palmitic acid or oleic acid, whereas the PTE1 mutant grew slower and to a lower density on oleic acid but not on palmitic acid. The identification of 3,5-tetradecadienoic acid in the medium of wild-type cells but not in the medium of the PTE1 mutant proves the operation of the thioesterase-dependent pathway of oleate beta-oxidation in S. cerevisiae. Dienoyl-CoA isomerase activity was very low in wild-type cells, fourfold higher in the YOR180c mutant, and not associated with purified Yor180c protein. These observations support the conclusion that the YOR180c gene does not encode dienoyl-CoA isomerase.
通过比较野生型细胞在油酸或棕榈酸上的生长情况与YOR180c（DCI1）基因缺失的突变体（据报道该基因编码Δ3,5、Δ2,4 - 二烯酰辅酶A异构酶（二烯酰辅酶A异构酶））或编码过氧化物酶体硫酯酶1的PTE1基因缺失的突变体的生长情况，研究了酿酒酵母中油酸的β - 氧化。野生型细胞在棕榈酸或油酸上的生长与YOR180c突变体细胞的生长没有区别，而PTE1突变体在油酸上生长较慢且密度较低，但在棕榈酸上则不然。在野生型细胞培养基中鉴定出3,5 - 十四碳二烯酸，而在PTE1突变体培养基中未鉴定出，这证明了酿酒酵母中硫酯酶依赖性油酸β - 氧化途径的运行。野生型细胞中的二烯酰辅酶A异构酶活性非常低，在YOR180c突变体中高四倍，并且与纯化的Yor180c蛋白无关。这些观察结果支持YOR180c基因不编码二烯酰辅酶A异构酶的结论。
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