Nakazawa Kumiko, Murata Shin-ichi, Yuminamochi Tsutomu, Ishii Yoshio, Ohno Shikine, Nakazawa Tadao, Kondo Tetsuo, Katoh Ryohei
Department of Pathology, University of Hospital, Faculty of Medicine, University of Yamanashi, Yamanashi 409-3898, Japan.
Am J Clin Pathol. 2009 Nov;132(5):776-84. doi: 10.1309/AJCP61KNVHJVHAFN.
We immunochemically studied p16(INK4a) expression in 116 urine cytologic samples and compared results with 190 histologic samples. The cytologic samples were classified into 4 groups: 1, mild cellular atypia; 2, moderate cellular atypia; 3, severe cellular atypia; and 4, malignancy. Overexpression of p16(INK4a) was detected in none of 32 cases in group 1, 8 (16%) of 50 cases in group 2, 5 (42%) of 12 cases in group 3, and 11 (50%) of 22 cases in group 4. In addition, by histologic analysis, p16(INK4a) overexpression was not detected in nonneoplastic urothelium, except for a few cases of reactive atypia, but it was detected in about 50% of urothelial carcinomas. In particular, a high incidence (16/20 [80%]) of p16(INK4a) overexpression in high-grade carcinomas was noted in cytologic samples. Immunocytologic analysis of p16(INK4a) expression in cytologic samples is a useful ancillary tool for detection of urothelial carcinoma with infiltrating potential.
我们对116份尿液细胞学样本中的p16(INK4a)表达进行了免疫化学研究，并将结果与190份组织学样本进行了比较。细胞学样本分为4组：1，轻度细胞异型性；2，中度细胞异型性；3，重度细胞异型性；4，恶性肿瘤。第1组32例中未检测到p16(INK4a)过表达，第2组50例中有8例(16%)，第3组12例中有5例(42%)，第4组22例中有11例(50%)。此外，通过组织学分析，除少数反应性异型性病例外，在非肿瘤性尿路上皮中未检测到p16(INK4a)过表达，但在约50%的尿路上皮癌中检测到。特别是，在细胞学样本中，高级别癌中p16(INK4a)过表达的发生率很高(20例中有16例[80%])。对细胞学样本中p16(INK4a)表达进行免疫细胞学分析是检测具有浸润潜能的尿路上皮癌的一种有用的辅助工具。
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