Yonezawa Tomo, Haga Satoshi, Kobayashi Yosuke, Katoh Kazuo, Obara Yoshiaki
Department of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Amamiyamachi, Sendai 981-8555, Japan.
Biochem Biophys Res Commun. 2009 Dec 18;390(3):915-9. doi: 10.1016/j.bbrc.2009.10.077. Epub 2009 Oct 21.
Brain-specific uncoupling proteins such as uncoupling protein 4 (UCP4) and brain mitochondrial carrier protein 1 (BMCP1; also known as UCP5) were identified by computational analysis for expressed sequence tag and hybridization screening. Both were detected at the mRNA level by RT-PCR in cloned bovine mammary epithelial cells (bMEC) and lactating bovine mammary glands. Physiological concentrations of saturated fatty acids (stearate and palmitate), but not unsaturated fatty acids (oleate and linoleate), induced up-regulation of BMCP1 mRNA in bMEC. Treatment with insulin induced down-regulation of UCP4 and BMCP1. These results suggest that UCP4 and BMCP1 are regulated by insulin and/or fatty acids in mammary epithelial cells and lactating mammary glands, and thereby may play an important role in lipid and energy metabolism.
通过对表达序列标签的计算分析和杂交筛选，鉴定出了脑特异性解偶联蛋白，如解偶联蛋白4（UCP4）和脑线粒体载体蛋白1（BMCP1；也称为UCP5）。通过RT-PCR在克隆的牛乳腺上皮细胞（bMEC）和泌乳牛乳腺中均在mRNA水平检测到了这两种蛋白。饱和脂肪酸（硬脂酸和棕榈酸）的生理浓度可诱导bMEC中BMCP1 mRNA的上调，但不饱和脂肪酸（油酸和亚油酸）则不能。胰岛素处理可诱导UCP4和BMCP1的下调。这些结果表明，UCP4和BMCP1在乳腺上皮细胞和泌乳乳腺中受胰岛素和/或脂肪酸的调节，因此可能在脂质和能量代谢中发挥重要作用。
