巯基和二硫键蛋白质：III. 天然蛋白质-血红蛋白和晶状体蛋白质的巯基。
SULFHYDRYL AND DISULFIDE GROUPS OF PROTEINS : III. SULFHYDRYL GROUPS OF NATIVE PROTEINS-HEMOGLOBIN AND THE PROTEINS OF THE CRYSTALLINE LENS.
机构信息
Hospital of The Rockefeller Institute for Medical Research, New York, and the Laboratories of The Rockefeller Institute for Medical Research, Princeton, N. J.
出版信息
J Gen Physiol. 1936 Jan 20;19(3):439-50. doi: 10.1085/jgp.19.3.439.
Hemoglobin and the proteins of the crystalline lens contain active SH groups while in the native state, the number of active groups increasing as the pH rises. All the SH groups of denatured globin and of the denatured lens proteins are active at a pH so low that practically none of the SH groups of native hemoglobin and of native lens protein are active. The effect of denaturation on the SH groups of a protein is to extend towards the acid side the pH range of their activity. It is possible to oxidize the iron-porphyrin and the SH groups of hemoglobin independently of each other.
血红蛋白和晶状体蛋白中的蛋白质在天然状态下含有活性 SH 基团，随着 pH 值的升高，活性基团的数量增加。在 pH 值如此低的情况下，变性球蛋白和变性晶状体蛋白的所有 SH 基团都是活跃的，而天然血红蛋白和天然晶状体蛋白的 SH 基团几乎没有一个是活跃的。变性对蛋白质中 SH 基团的影响是将其活性的 pH 范围扩展到酸性侧。可以独立地氧化血红素和血红蛋白的 SH 基团。
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