Department of Endocrinology, Institute of Endocrinology & Metabolism, Anhui Medical University, Anhui, China.
Endocr Res. 2009;34(4):130-41. doi: 10.3109/07435800903287061.
To investigate the effect of pioglitazone on visfatin expression.
We studied the effect of pioglitazone on visfatin expression in 3T3-L1 adipocytes and serum concentrations and tissue expression of visfatin in normal Sprague-Dawley rats and rats with insulin resistance induced by high-fat diet (HF). Metabolic and anatomical parameters of the rats were also performed.
In 3T3-L1 adipocytes, visfatin expression increased during the differentiation and it was not regulated by pioglitazone. In the rats, 12 weeks of HF feeding induced obesity and increased fast blood glucose (FBG), serum insulin and circulating visfatin. Pioglitazone treatment ameliorated insulin resistance with concomitant reduction in serum visfatin, free fatty acid, and triglyceride (TG) of the rats fed HF. Compared with subcutaneous adipose tissue and muscle, visfatin protein expression was much higher in visceral adipose tissue on both diets (p < 0.05 for all). Visfatin expression decreased in visceral adipose tissue but not subcutaneous adipose tissue or muscle after pioglitazone treatment in HF-fed rats. Visfatin expression in the rats fed chow diet was not affected by pioglitazone. Additionally, we demonstrated that serum visfatin was positively correlated with visceral adipose tissue weight, visfatin in visceral adipose tissue, TG and FBG (p < 0.05 for all).
Visfatin is preferentially produced by visceral fat and peroxisome proliferator-activated receptor-gamma agonist ameliorates the development of insulin resistance in HF-fed rats with a major decrease in visfatin expression, the effect of pioglitazone on visfatin in HF-fed rats is dependent on glucose and lipid metabolism in the animals.
研究吡格列酮对内脂素表达的影响。
我们研究了吡格列酮对 3T3-L1 脂肪细胞内脂素表达的影响，以及正常 Sprague-Dawley 大鼠和高脂肪饮食（HF）诱导胰岛素抵抗大鼠血清内脂素浓度和组织表达。还对大鼠的代谢和解剖参数进行了研究。
在 3T3-L1 脂肪细胞中，内脂素表达在分化过程中增加，不受吡格列酮调节。在大鼠中，12 周的 HF 喂养导致肥胖，并增加了快速血糖（FBG）、血清胰岛素和循环内脂素。吡格列酮治疗改善了胰岛素抵抗，同时降低了 HF 喂养大鼠的血清内脂素、游离脂肪酸和甘油三酯（TG）。与皮下脂肪组织和肌肉相比，两种饮食中内脏脂肪组织的内脂素蛋白表达都高得多（p < 0.05）。在 HF 喂养的大鼠中，吡格列酮治疗后，内脏脂肪组织中的内脂素表达降低，但皮下脂肪组织和肌肉中的内脂素表达没有降低。在给予正常饮食的大鼠中，吡格列酮对内脂素表达没有影响。此外，我们还证明了血清内脂素与内脏脂肪组织重量、内脏脂肪组织中的内脂素、TG 和 FBG 呈正相关（p < 0.05）。
内脂素主要由内脏脂肪组织产生，过氧化物酶体增殖物激活受体-γ 激动剂改善 HF 喂养大鼠胰岛素抵抗的发展，主要通过降低内脂素表达，吡格列酮对 HF 喂养大鼠内脂素的作用依赖于动物的糖脂代谢。
