激活素抑制人视网膜母细胞瘤Y79细胞的生长并诱导其分化。

Activin inhibits cell growth and induces differentiation in human retinoblastoma y79 cells.

作者信息

Kanno Chikako, Kashiwagi Yoshiko, Horie Kuniko, Inomata Motoko, Yamamoto Teiko, Kitanaka Chifumi, Yamashita Hidetoshi

机构信息

Department of Ophthalmology and Visual Science, Yamagata University School of Medicine, Yamagata, Japan.

出版信息

Curr Eye Res. 2009 Aug;34(8):652-9. doi: 10.1080/02713680903007147.

DOI:10.1080/02713680903007147

PMID:19899992

Abstract

PURPOSE

Activin is a member of the transforming growth factor-beta (TGF-beta) superfamily and exerts certain effects on differentiation and apoptosis. We investigated the effects of activin on retinoblastoma cell line.

MATERIALS AND METHODS

We used retinoblastoma cell line Y79. Intracellular signal transduction of activin was investigated with RT-PCR, immunofluorescence study, and luciferase reporter assay. The effect of activin on cell growth was examined with fluorescence cell viability assays. To determine the effect of activin on apoptosis, a TUNEL assay and an immunofluorescence study of cleaved PARP were performed. The effect of activin on cell differentiation was examined with RT-PCR and Western blotting.

RESULTS

Intracellular signal transduction of activin was confirmed in Y79 cells. Activin inhibited Y79 cell growth. Activin induced the expression of neural retina leucine zipper (Nrl) at the mRNA and protein levels.

CONCLUSIONS

Nrl is a specific gene in rod photoreceptor development and is a gene indispensable to differentiation into rod photoreceptors, so the present results suggest that activin affects the differentiation of retinoblastoma cells into rod photoreceptor cells.

摘要

目的

激活素是转化生长因子-β（TGF-β）超家族的成员，对细胞分化和凋亡具有一定作用。我们研究了激活素对视网膜母细胞瘤细胞系的影响。

材料与方法

我们使用了视网膜母细胞瘤细胞系Y79。通过逆转录-聚合酶链反应（RT-PCR）、免疫荧光研究和荧光素酶报告基因检测来研究激活素的细胞内信号转导。用荧光细胞活力检测法检测激活素对细胞生长的影响。为了确定激活素对凋亡的影响，进行了末端脱氧核苷酸转移酶介导的缺口末端标记（TUNEL）检测和对裂解的聚（ADP-核糖）聚合酶（PARP）的免疫荧光研究。用RT-PCR和蛋白质印迹法检测激活素对细胞分化的影响。