马来西亚两种常见丽蝇物种，大头金蝇（Chrysomya megacephala (Fabricius)）和红尾肉蝇（Chrysomya rufifacies (Macquart)）（双翅目：丽蝇科）细胞色素氧化酶亚基I和II基因的序列变异。

Sequence variation in the cytochrome oxidase subunit I and II genes of two commonly found blow fly species, Chrysomya megacephala (Fabricius) and Chrysomya rufifacies (Macquart) (Diptera: Calliphoridae) in Malaysia.

作者信息

Tan Siew Hwa, Aris Edah Mohd, Surin Johari, Omar Baharudin, Kurahashi Hiromu, Mohamed Zulqarnain

机构信息

Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.

出版信息

Trop Biomed. 2009 Aug;26(2):173-81.

PMID:19901904

Abstract

The mitochondiral DNA region encompassing the cytochrome oxidase subunit I (COI) and cytochrome oxidase subunit II (COII) genes of two Malaysian blow fly species, Chrysomya megacephala (Fabricius) and Chrysomya rufifacies (Macquart) were studied. This region, which spans 2303bp and includes the COI, tRNA leucine and partial COII was sequenced from adult fly and larval specimens, and compared. Intraspecific variations were observed at 0.26% for Ch. megacephala and 0.17% for Ch. rufifacies, while sequence divergence between the two species was recorded at a minimum of 141 out of 2303 sites (6.12%). Results obtained in this study are comparable to published data, and thus support the use of DNA sequence to facilitate and complement morphology-based species identification.

摘要

对两种马来西亚丽蝇——大头金蝇（Chrysomya megacephala，Fabricius）和红头丽蝇（Chrysomya rufifacies，Macquart）中包含细胞色素氧化酶亚基I（COI）和细胞色素氧化酶亚基II（COII）基因的线粒体DNA区域进行了研究。该区域跨度为2303bp，包括COI、亮氨酸转运RNA（tRNA leucine）和部分COII，对成年蝇和幼虫样本进行了测序并比较。大头金蝇的种内变异率为0.26%，红头丽蝇为0.17%，而两个物种之间的序列差异在2303个位点中至少有141个（6.12%）。本研究获得的结果与已发表的数据相当，因此支持使用DNA序列来促进和补充基于形态学的物种鉴定。