Bahari Ali, Aarabi Mahmoud, Aarabi Mohsen, Hedayati Mehdi, Jarollahi Ali, Firouzi Farzad, Aghazadeh Rahim, Zali Mohammad Reza, Hashemi Mohammad
Department of Gastroenterology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Acta Gastroenterol Belg. 2009 Jul-Sep;72(3):301-5.
Perinuclear antineutrophil cytoplasmic autoantibodies (pANCA) and anti-Saccharomyces Cerevisiae antibody (ASCA) are potential markers for diagnosis of inflammatory bowel disease (IBD). The aim of the present study was to evaluate the diagnostic value of pANCA and ASCA in Iranian patients with IBD.
Serum samples were collected from 144 patients with IBD (113 ulcerative colitis and 31 Crohn's disease) and patients with non-IBD problems were assayed for ASCA by Enzyme-Linked Immunosorbent Assay (ELISA) and for pANCA by indirect immunofluorescence assay.
Sensitivity and specificity of pANCA in UC were 39.8% and 82.1%, respectively. For CD, pASCA test provided the sensitivity of 58% and specificity of 70%. A combination of pANCA+/ASCA- for diagnosis of UC showed a sensitivity of 31.9% and specificity of 89.1%. In addition the combination of pANCA-/ASCA+ showed a sensitivity of 35.5% and specificity of 79.8% for diagnosis of CD.
Due to low sensitivity of pANCA and ASCA alone or in combination, they are not valuable serological markers for diagnosis of UC or CD.
核周型抗中性粒细胞胞浆抗体（pANCA）和抗酿酒酵母抗体（ASCA）是诊断炎症性肠病（IBD）的潜在标志物。本研究旨在评估pANCA和ASCA在伊朗IBD患者中的诊断价值。
收集了144例IBD患者（113例溃疡性结肠炎和31例克罗恩病）的血清样本，采用酶联免疫吸附测定（ELISA）检测非IBD问题患者的ASCA，采用间接免疫荧光测定法检测pANCA。
pANCA在UC中的敏感性和特异性分别为39.8%和82.1%。对于CD，pASCA检测的敏感性为58%，特异性为70%。pANCA+/ASCA-联合诊断UC的敏感性为31.9%，特异性为89.1%。此外，pANCA-/ASCA+联合诊断CD的敏感性为35.5%，特异性为79.8%。
由于pANCA和ASCA单独或联合使用时敏感性较低，它们不是诊断UC或CD有价值的血清学标志物。
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