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无血清、无动物成分的培养基可维持脂肪干细胞在体外的增殖率和多能性。

Serum-free, xeno-free culture media maintain the proliferation rate and multipotentiality of adipose stem cells in vitro.

机构信息

University of Tampere and Tampere University Hospital, Regea Institute for Regenerative Medicine, Tampere, Finland.

出版信息

Cytotherapy. 2009;11(7):958-72. doi: 10.3109/14653240903233081.

Abstract

BACKGROUND AIMS

Human adipose stem cells (ASC) are an abundant, readily available population of multipotent progenitor cells that reside in adipose tissue. ASC have been shown to have therapeutic applicability in pre-clinical studies, but a standardized expansion method for clinical cell therapy has yet to be established. Isolated ASC are typically expanded in medium containing fetal bovine serum (FBS); however, sera and other culturing reagents of animal origin in clinical therapy pose numerous safety issues, including possible infections and severe immune reactions.

METHODS

To identify optimal conditions for ex vivo expansion of ASC, the effects of seven serum-free (SF) and xeno-free (XF) media were investigated with both FBS and allogeneic human serum (alloHS; as a control media). Surface marker expression, proliferation, morphology and differentiation analyzes were utilized for investigating the effects of media on ASC.

RESULTS

The proliferation and morphology analysis demonstrated significant differences between ASC cultured in SF/XF culture media compared with serum-containing culture media, with medium prototype StemPro MSC SFM XenoFree providing significantly higher proliferation rates than ASC cultured in media containing serum, while still maintaining the differentiation potential and surface marker expression profile characteristic of ASC.

CONCLUSIONS

Looking forward, fully defined XF media formulations will provide the means for the development and approval of safer clinical cell therapy treatments. However, to fully recognize the capacity of these XF culture media, further pre-clinical safety and efficacy studies must be performed.

摘要

背景目的

人类脂肪干细胞(ASC)是一种丰富、易于获得的多能祖细胞群,存在于脂肪组织中。ASC 在临床前研究中已被证明具有治疗应用价值,但尚未建立用于临床细胞治疗的标准化扩增方法。分离的 ASC 通常在含有胎牛血清(FBS)的培养基中扩增;然而,临床治疗中血清和其他动物来源的培养试剂存在许多安全问题,包括可能的感染和严重的免疫反应。

方法

为了确定 ASC 体外扩增的最佳条件,研究了七种无血清(SF)和无动物源(XF)培养基对 FBS 和同种异体人血清(alloHS;作为对照培养基)的影响。利用表面标志物表达、增殖、形态和分化分析来研究培养基对 ASC 的影响。

结果

与含血清的培养基相比,SF/XF 培养基中 ASC 的增殖和形态分析显示出显著差异,原型培养基 StemPro MSC SFM XenoFree 提供的增殖率明显高于含血清的培养基中培养的 ASC,同时仍保持 ASC 的分化潜能和表面标志物表达特征。

结论

展望未来,完全定义的 XF 培养基配方将为开发和批准更安全的临床细胞治疗提供手段。然而,要充分认识这些 XF 培养基的能力,还必须进行进一步的临床前安全性和疗效研究。

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