School of Life Science, Wuhan University of Science and Technology Zhongnan Branch, Wuhan 430223, China.
J Biosci Bioeng. 2009 Dec;108(6):496-500. doi: 10.1016/j.jbiosc.2009.06.001.
The dye-decolorizing potential of bilirubin oxidase (BOX) was demonstrated for an anthraquinone dye, remazol brilliant blue R (RBBR). The dye was decolorized 40% within 4 h by the BOX alone, whereas it was more efficient in the presence of 2, 2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), showing 91.5% decolorization within 25 min. The effects of operational parameters on decolorization were examined. The results showed that the decolorization efficiency decreased with increasing RBBR concentration, and a marked inhibition effect was exhibited when the dye concentrations were above 100 mg l(-1). The optimum temperature for enzymatic decolorization was 40 degrees C. BOX showed efficient decolorization of the dye with a wide pH range of 5-8.5. The maximum decolorization activity occurred at pH 8 with ABTS and at pH 5 without ABTS. Analysis of RBBR ultraviolet and visible (UV-VIS) spectra after BOX treatment indicated that the decolorization of RBBR was due to biodegradation. Our results suggested that ABTS can serve as an electron mediator to facilitate the oxidation of RBBR, and the BOX-ABTS mediator-involved dye decolorization mechanism was similar to that of laccase. Operation over a wide range of pH and efficient decolorization suggested that the BOX can be used to decolorize synthetic dyes from effluents, especially for anthraquinonic dyes.
胆红素氧化酶（BOX）具有染料脱色能力，本研究以蒽醌染料活性艳蓝 RBBR 为处理对象，结果表明，BOX 单独作用 4 h 可使染料脱色 40%，而当体系中存在 2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）时，染料的脱色更为高效，25 min 内即可达到 91.5%的脱色率。考察了操作参数对脱色效果的影响，结果表明，随着 RBBR 浓度的增加，BOX 的脱色效率逐渐降低，当染料浓度高于 100 mg/L 时，BOX 的脱色效果受到明显抑制。酶促脱色的最适温度为 40°C。BOX 在 pH 5-8.5 的宽范围内均具有高效的脱色能力。当使用 ABTS 作为电子介体时，BOX 的最大脱色活性出现在 pH 8，而当不使用 ABTS 时，最大脱色活性出现在 pH 5。BOX 处理 RBBR 前后的紫外-可见（UV-VIS）光谱分析表明，RBBR 的脱色是由于生物降解所致。我们的结果表明，ABTS 可以作为电子介体促进 RBBR 的氧化，BOX-ABTS 介体参与的染料脱色机制与漆酶相似。BOX 可在较宽的 pH 范围内运行并实现高效的染料脱色，这表明其可用于处理废水中的合成染料，尤其适用于蒽醌类染料。
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