SEPT7基因的上调抑制人胶质瘤细胞的侵袭。

Upregulation of SEPT7 gene inhibits invasion of human glioma cells.

作者信息

Xu Song, Jia Zhi-Fan, Kang Chunsheng, Huang Qiang, Wang Guangxiu, Liu Xiaozhi, Zhou Xuan, Xu Peng, Pu Peiyu

机构信息

Laboratory of Neuro-Oncology, Tianjin Neurological Institute, Tianjin Medical University General Hospital, Tianjin 300052, People's Republic of China.

出版信息

Cancer Invest. 2010 Mar;28(3):248-58. doi: 10.3109/07357900903179609.

DOI:10.3109/07357900903179609

PMID:19916744

Abstract

OBJECTIVES

To explore the role of SEPT7 in glioma cell invasion.

METHODS

SEPT7 was transfected into human glioma cell lines U251 and TJ899, the invasive abilities were evaluated by transwell assay, scratch assay, and 3-D/2-D Matrigel growth. The expression of MMP2/9, MT1-MMP, integrin alpha(v)beta(3), and TIMP1/2 was detected by immunohistochemistry, immunofluorescence, and Western blot analyses. Distribution of alpha-tubulin was examined by laser scanning confocal analysis.

RESULT

After SEPT7 trasfection, cell invasion was inhibited, expression of MMP2/9, MT1-MMP, and integrin alpha(v)beta(3) was decreased, while TIMP1/2 was increased, and alpha-tubulin was redistributed.

CONCLUSION

These results suggest that SEPT7 plays an important role in the glioma cell invasion.

摘要

目的

探讨SEPT7在胶质瘤细胞侵袭中的作用。

方法

将SEPT7转染到人胶质瘤细胞系U251和TJ899中，通过Transwell实验、划痕实验和三维/二维基质胶生长实验评估侵袭能力。采用免疫组织化学、免疫荧光和蛋白质免疫印迹分析检测基质金属蛋白酶2/9（MMP2/9）、膜型基质金属蛋白酶1（MT1-MMP）、整合素α(v)β3和金属蛋白酶组织抑制因子1/2（TIMP1/2）的表达。通过激光扫描共聚焦分析检测α-微管蛋白的分布。