[利用GK大鼠建立新型胃旁路动物模型]

[Establishment of a new gastric bypass animal-model with GK rats].

作者信息

Sun Qing-lei, Wang Yu, Zhang Xin-guo, Gao Hong-kai, Zhao Liang

机构信息

Department of General Surgery, The Affiliated Hospital of Binzhou Medical College, Shandong Binzhou 256603, China.

出版信息

Zhonghua Wei Chang Wai Ke Za Zhi. 2009 Nov;12(6):562-4.

PMID:19921563

Abstract

OBJECTIVE

To establish a new gastric bypass animal-model with Goto-Kakizaki rats whose different parts of the small intestine were bypassed while stomach was not bypassed.

METHODS

Forty male 3-month-old GK rats were randomly divided into 4 groups: group I (sham operation), group II (duodenum bypassed), group III (jejunum bypassed), group IV (ileum bypassed). Fasting plasma glucose was measured before operation and the 1st, 4th,and 8th week after operation in all the rats, the body weight of all the rats were measured simultaneously.

RESULTS

The survival rate of operation for the rats was 95%. Two rats in group IV (died on the first day after operation. The mean fasting plasma glucose concentration of the rats in group II, III, IV (declined obviously 4 weeks after gastric bypass [group II (12.02+/-1.97) vs (6.36+/-0.50) mmol/L, group III (13.42+/-1.66) vs (5.96+/-0.53) mmol/L, group IV (14.32+/-2.82) vs (5.18+/-0.49) mmol/L, all P <0.01], but there were no significant differences among the gastric bypassed groups. The weight of rats in group I, II, III (increased obviously after gastric bypass [group I (253.6+/-9.37) vs (367.0+/-23.70) g, group II (268.2+/-7.95) vs (384.8+/-16.12) g, group III (253.0+/-6.20) vs (323.0+/-16.40) g, all P <0.05] except the rats in group IV ([(262.0+/-13.47) vs(185.8+/-11.56) g].

CONCLUSIONS

The mean fasting plasma glucose concentration of the GK rats decreases obviously after gastric bypass through different parts of small intestine. The fasting plasma glucose concentration is not associated with the length of small intestine and body weight.

摘要

目的

利用Goto-Kakizaki大鼠建立一种新的胃旁路动物模型，该模型不绕过胃，而是绕过小肠的不同部位。

方法

将40只3月龄雄性GK大鼠随机分为4组：Ⅰ组（假手术组）、Ⅱ组（十二指肠旁路组）、Ⅲ组（空肠旁路组）、Ⅳ组（回肠旁路组）。测量所有大鼠术前及术后第1、4、8周的空腹血糖，同时测量所有大鼠的体重。

结果

大鼠手术存活率为95%。Ⅳ组有2只大鼠（术后第1天死亡）。Ⅱ组、Ⅲ组、Ⅳ组大鼠胃旁路术后4周空腹血糖平均浓度明显下降[Ⅱ组（12.02±1.97）对（6.36±0.50）mmol/L，Ⅲ组（13.42±1.66）对（5.96±0.53）mmol/L，Ⅳ组（14.32±2.82）对（5.18±0.49）mmol/L，均P<0.01]，但胃旁路组之间无显著差异。Ⅰ组、Ⅱ组、Ⅲ组大鼠胃旁路术后体重明显增加[Ⅰ组（253.6±9.37）对（367.0±23.70）g，Ⅱ组（268.2±7.95）对（384.8±16.12）g，Ⅲ组（253.0±6.20）对（323.0±16.40）g，均P<0.05]，Ⅳ组大鼠除外[（262.0±13.47）对（185.8±11.56）g]。