School of Pharmacy, Showa University, Tokyo 142-8555, Japan.
Luminescence. 2010 Nov-Dec;25(6):456-62. doi: 10.1002/bio.1180. Epub 2009 Nov 18.
Nitric oxide (NO) is related to various physiological effects as well as to numerous diseases caused by accentuation of NO production. Measurement of NO in cells and tissues is difficult as NO readily reacts with other molecules; furthermore, its half-life as a radical is fleeting. Currently, many NO pharmaceuticals are marketed as therapeutic agents for ischemic disease. Consequently, the identification of NO radicals and determination of generation rate from pharmaceuticals is very important when the effect of the medicinal supply is estimated. In this study, we developed a fluorometric assay for NO employing sesamol (3,4-methylenedioxyphenol) as a fluorometric substrate. Sesamol is converted to a fluorescent derivative (ex. 365 nm, em. 447 nm), which is dimmer in the presence of NO. The detection limit of NO with this method is 400 fmol; moreover, NO generated from drugs can be measured.
一氧化氮（NO）与多种生理效应有关，也与许多由 NO 产生加剧引起的疾病有关。由于 NO 很容易与其他分子反应，因此测量细胞和组织中的 NO 非常困难；此外，其作为自由基的半衰期非常短暂。目前，许多 NO 药物作为治疗缺血性疾病的药物上市。因此，当估计药物供应的效果时，识别 NO 自由基并确定其从药物中的生成速率非常重要。在这项研究中，我们开发了一种使用芝麻酚（3,4-亚甲二氧基苯酚）作为荧光底物的 NO 荧光测定法。芝麻酚转化为荧光衍生物（激发 365nm，发射 447nm），在存在 NO 的情况下其荧光强度降低。该方法检测 NO 的检测限为 400fmol；此外，还可以测量药物产生的 NO。
