猫潜在性猫白血病病毒感染在非再生性血细胞减少症中的作用。

Role of latent feline leukemia virus infection in nonregenerative cytopenias of cats.

机构信息

Clinic of Small Animal Medicine, LMU University of Munich, Munich, Germany.

出版信息

J Vet Intern Med. 2010 Jan-Feb;24(1):192-7. doi: 10.1111/j.1939-1676.2009.0417.x.

DOI:10.1111/j.1939-1676.2009.0417.x

PMID:19925574

Abstract

BACKGROUND

Nonregenerative cytopenias such as nonregenerative anemia, neutropenia, and thrombocytopenia in cats with feline leukemia virus (FeLV) antigen are assumed to be caused by the underlying FeLV infection. In addition, cats with negative FeLV antigen-test results that have cytopenias of unknown etiology often are suspected to suffer from latent FeLV infection that is responsible for the nonregenerative cytopenias.

OBJECTIVE

The purpose of this study was to assess the role of latent FeLV infection by polymerase chain reaction (PCR) in bone marrow of cats with nonregenerative cytopenias that had negative FeLV antigen test results in blood.

ANIMALS

Thirty-seven cats were included in the patient group. Inclusion criteria were (1) nonregenerative cytopenia of unknown origin and (2) negative FeLV antigen test result. Antigenemia was determined by detection of free FeLV p27 antigen by ELISA in serum. Furthermore, 7 cats with positive antigen test results with nonregenerative cytopenia were included as control group I, and 30 cats with negative antigen test results without nonregenerative cytopenia were included as control group II.

METHODS

Whole blood and bone marrow samples were tested by 2 different PCR assays detecting sequences of the envelope or long terminal repeat genes. FeLV immunohistochemistry was performed in bone marrow samples.

RESULTS

Two of the 37 cats (5.4%) in the patient group were positive on the bone marrow PCR results and thus were latently infected with FeLV.

CONCLUSIONS AND CLINICAL IMPORTANCE

The findings of this study suggest that FeLV latency is rare in cats with nonregenerative cytopenias.

摘要

背景

猫白血病病毒 (FeLV) 抗原阳性的猫出现非再生性血细胞减少症（如非再生性贫血、中性粒细胞减少症和血小板减少症），被认为是由潜在的 FeLV 感染引起的。此外，对 FeLV 抗原检测结果为阴性且出现不明原因血细胞减少症的猫，常被怀疑患有潜伏性 FeLV 感染，这种感染是导致非再生性血细胞减少症的原因。

目的

本研究旨在通过聚合酶链反应 (PCR) 评估潜伏性 FeLV 感染在骨髓中的作用，这些骨髓来自 FeLV 抗原检测结果为阴性但血液中存在非再生性血细胞减少症的猫。

动物

37 只猫被纳入患者组。纳入标准为：（1）病因不明的非再生性血细胞减少症；（2）FeLV 抗原检测结果为阴性。通过酶联免疫吸附试验（ELISA）检测血清中的游离 FeLV p27 抗原来确定抗原血症。此外，还纳入了 7 只抗原检测结果为阳性且伴有非再生性血细胞减少症的猫作为对照组 I，纳入了 30 只抗原检测结果为阴性且无非再生性血细胞减少症的猫作为对照组 II。