Institute of Food Engineering, College of Life & Environment Science, Shanghai Normal University, Shanghai, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2010 Jan;75(1):299-304. doi: 10.1016/j.saa.2009.10.027. Epub 2009 Oct 21.
There are several models by means of quenching fluorescence of BSA to determine the binding parameters. The binding parameters obtained from different models are quite different from each other. Which model is suitable to study the interaction between trans-resveratrol and BSA? Herein, twelve models based fluorescence quenching of BSA were compared. The number of binding sites increasing with increased binding constant for similar compounds binding to BSA maybe one approach to resolve this question. For example, here eleven flavonoids were tested to illustrate that the double logarithm regression curve is suitable to study binding polyphenols to BSA.
有几种通过猝灭 BSA 荧光来确定结合参数的模型。从不同模型获得的结合参数彼此之间有很大的不同。哪种模型适合研究反式白藜芦醇与 BSA 的相互作用?在此,比较了基于 BSA 荧光猝灭的 12 种模型。对于类似化合物与 BSA 的结合,结合常数增加时结合位点的数量增加,这可能是解决这个问题的一种方法。例如,在这里测试了 11 种类黄酮来说明双对数回归曲线适合研究结合多酚与 BSA。