Fabrication and evaluation of a ratchet type dielectrophoretic device for particle analysis.

Dielectrophoresis is an electrokinetic phenomenon that utilizes an asymmetric electric field to separate analytes based on differences in their polarizabilities relative to that of the suspending medium. One dielectrophoretic device architecture that offers interesting possibilities for particle transport without the use of external flow is the ratchet geometry. This paper describes the fabrication and evaluation of a novel dielectrophoretic ratchet device using a series of fine particles as test probes. The asymmetrical electric field required to selectively transport target analytes was produced using electroformed electrodes which offer the possibility of reducing convective heating and which can be used to construct a device in which all particles located within the fluidic channel are exposed to the applied field. Initial tests of this device were conducted using magnetite and polystyrene fine particles to demonstrate selective particle collection and a separation based on differences in the electrical properties of the analytes employed.