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棘轮式介电泳粒子分析装置的制作与评价。

Fabrication and evaluation of a ratchet type dielectrophoretic device for particle analysis.

机构信息

Department of Chemistry, Oregon State University, Corvallis, OR 97331-4003, USA.

出版信息

J Chromatogr A. 2009 Dec 25;1216(52):9063-70. doi: 10.1016/j.chroma.2009.10.078. Epub 2009 Oct 30.

Abstract

Dielectrophoresis is an electrokinetic phenomenon that utilizes an asymmetric electric field to separate analytes based on differences in their polarizabilities relative to that of the suspending medium. One dielectrophoretic device architecture that offers interesting possibilities for particle transport without the use of external flow is the ratchet geometry. This paper describes the fabrication and evaluation of a novel dielectrophoretic ratchet device using a series of fine particles as test probes. The asymmetrical electric field required to selectively transport target analytes was produced using electroformed electrodes which offer the possibility of reducing convective heating and which can be used to construct a device in which all particles located within the fluidic channel are exposed to the applied field. Initial tests of this device were conducted using magnetite and polystyrene fine particles to demonstrate selective particle collection and a separation based on differences in the electrical properties of the analytes employed.

摘要

介电泳是一种电动现象,利用不对称电场根据与悬浮介质相比的介电常数差异来分离分析物。一种在不使用外部流动的情况下提供有趣的粒子传输可能性的介电泳装置结构是棘轮几何形状。本文描述了使用一系列细颗粒作为测试探针的新型介电泳棘轮装置的制造和评估。使用电成型电极产生所需的不对称电场,以选择性地输送目标分析物,电成型电极具有降低对流加热的可能性,并且可以用于构建一种装置,其中位于流道内的所有颗粒都暴露于施加的电场。该装置的初步测试使用磁铁矿和聚苯乙烯细颗粒进行,以证明基于所使用的分析物的电特性的选择性颗粒收集和分离。

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