Department of Life Sciences, King's College London, Franklin Wilkins Building, London SE1 9NH, UK.
Syst Appl Microbiol. 2010 Jan;33(1):20-4. doi: 10.1016/j.syapm.2009.10.003. Epub 2009 Nov 22.
Analysis, using the polymerase chain reaction (PCR), restriction enzyme endonuclease analysis (REA), protein profile patterns, random amplification of polymorphic DNA (RAPD) fingerprinting, 16S rRNA gene sequencing and antisera growth inhibition tests, of 22 strains of Mycoplasma mycoides subsp. mycoides Large Colony type (MmmLC) and eight strains of M. mycoides subsp. capri (Mmc) are presented, along with a summary of comparative data from the literature for over 100 strains, all of which supports the reclassification of the MmmLC and Mmc strains into the single subspecies, M. mycoides subspecies capri.
使用聚合酶链反应(PCR)、限制性内切酶酶切分析(REA)、蛋白质图谱分析、随机扩增多态性 DNA(RAPD)指纹图谱、16S rRNA 基因测序和抗血清生长抑制试验,对 22 株绵羊肺炎支原体亚种大型菌落型(MmmLC)和 8 株山羊支原体亚种(Mmc)进行了分析,并对来自文献的 100 多株菌株的比较数据进行了总结,所有这些都支持将 MmmLC 和 Mmc 菌株重新分类为单一亚种,即绵羊肺炎支原体亚种 capri。