Han YouSheng, Luan FuLei, Zhu HongLiang, Shao Yi, Chen AnJun, Lu ChengWen, Luo YunBo, Zhu BenZhong
Laboratory of Fruit Biotechnology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
Sci China C Life Sci. 2009 Nov;52(11):1091-100. doi: 10.1007/s11427-009-0144-y. Epub 2009 Nov 24.
microRNAs (miRNAs) are a class of endogenous, non-coding, short (approximately 21 nt) RNAs directly involved in regulating gene expression at the post-transcriptional level. Previous reports have noted that plant miRNAs in the plant kingdom are highly conserved, which provides the foundation for identification of conserved miRNAs in other plant species through homology alignment. Conserved miRNAs in wheat are identified using EST (Expressed Sequence Tags) and GSS analysis. All previously known miRNAs in other plant species were blasted against wheat EST and GSS sequences to select novel miRNAs in wheat by a series of filtering criteria. From a total of 37 conserved miRNAs belonging to 18 miRNA families 10 conserved miRNAs comprising 4 families were reported in wheat. MiR395 is found to be a special family, because three members belonging to the same miR395 family are clustered together, similar to animal miRNAs. MiRNA targets are transcription factors involved in wheat growth and development, metabolism,and stress responses.
微小RNA（miRNA）是一类内源性、非编码的短（约21个核苷酸）RNA，直接参与转录后水平的基因表达调控。先前的报道指出，植物界中的植物miRNA高度保守，这为通过同源比对在其他植物物种中鉴定保守的miRNA奠定了基础。利用EST（表达序列标签）和GSS分析鉴定小麦中的保守miRNA。将其他植物物种中所有先前已知的miRNA与小麦EST和GSS序列进行比对，通过一系列筛选标准来选择小麦中的新miRNA。在小麦中报道了属于18个miRNA家族的37个保守miRNA中的10个保守miRNA，它们包含4个家族。发现miR395是一个特殊的家族，因为属于同一miR395家族的三个成员聚集在一起，类似于动物miRNA。miRNA靶标是参与小麦生长发育、代谢和应激反应的转录因子。
