Mississippi State University, Department of Biological Sciences at the College of Veterinary Medicine, Mississippi State, MS 39762, USA.
Can J Microbiol. 2009 Nov;55(11):1261-6. doi: 10.1139/w09-091.
The peptide antibiotic mutacin 1140 belongs to the group of antibiotics called lantibiotics. They are ribosomally synthesized and undergo extensive enzymatic modifications before being excreted into the culture medium. By using reverse-phase-high-performance liquid chromatography (RP-HPLC) and a semiquantitative bacteriocin bioassay to track mutacin 1140, an efficient ammonium sulfate (AS) precipitation method has been developed for removing mutacin 1140 from a complex medium containing 5% yeast extract. This method minimizes the amounts of fermentation by-products and media components that make downstream purification processes more difficult and economically infeasible. The method may be adaptable for the initial purification step of other lantibiotics. A threefold decrease in the precipitation of the medium components found in yeast extract, at pH 8.0 vs. pH 2.0, may have broad utility for the isolation of secondary metabolites produced in this complex medium. The average yield of mutacin 1140 from the fermentation medium was determined as 66%.
抗菌肽 mutacin 1140 属于一类被称为类脂肽的抗生素。它们是核糖体合成的，并在被分泌到培养基中之前经历广泛的酶修饰。通过使用反相高效液相色谱 (RP-HPLC) 和半定量细菌素生物测定法来跟踪 mutacin 1140，已经开发出一种有效的硫酸铵 (AS) 沉淀方法，用于从含有 5%酵母提取物的复杂培养基中去除 mutacin 1140。该方法最大限度地减少了发酵副产物和培养基成分的量，这些物质会使下游的纯化过程更加困难和在经济上不可行。该方法可能适用于其他类脂肽的初始纯化步骤。与 pH 2.0 相比，在 pH 8.0 下，酵母提取物中发现的培养基成分的沉淀减少了三倍，这可能对从这种复杂培养基中分离产生的次级代谢产物具有广泛的应用。从发酵培养基中获得的 mutacin 1140 的平均产量确定为 66%。
