Isolation of cutaneous dendritic cells.
作者信息
Helft Julie, Merad Miriam
机构信息
Department of Gene and Cell Medicine and The Immunology Institute, Mount Sinai School of Medicine, New York, NY, USA.
出版信息
Methods Mol Biol. 2010;595:231-3. doi: 10.1007/978-1-60761-421-0_15.
PMID:19941116
Abstract
Cutaneous dendritic cells represent the first immunological interface with the environment and play a key role in the defense against pathogens that breach the skin. This protocol describes how to isolate cutaneous dendritic cells from mouse ears for flow cytometry analysis and functional assay studies.
摘要
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