Department of Physiology, University of Illinois at Chicago, College of Medicine Research Building, Chicago, IL 60612, USA.
Small. 2010 Feb 5;6(3):355-60. doi: 10.1002/smll.200901757.
A small quantity of stiff microstructures in a 3D matrix regulates human mesenchymal stem cells to increase proliferation and slow differentiation after 10 days in culture. Cell morphology, clustering and gene expression are significantly different although the stiff microstructures are only 0.07% of the total gel volume.
在三维基质中少量的刚性微结构调节人骨髓间充质干细胞在培养 10 天后增加增殖并减缓分化。尽管刚性微结构仅占凝胶总体积的 0.07%,但细胞形态、聚集和基因表达有显著差异。